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| | <StructureSection load='2uyp' size='340' side='right'caption='[[2uyp]], [[Resolution|resolution]] 2.44Å' scene=''> | | <StructureSection load='2uyp' size='340' side='right'caption='[[2uyp]], [[Resolution|resolution]] 2.44Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2uyp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UYP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2uyp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UYP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2uyj|2uyj]], [[2uyk|2uyk]], [[2uyn|2uyn]]</div></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyp OCA], [https://pdbe.org/2uyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uyp RCSB], [https://www.ebi.ac.uk/pdbsum/2uyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyp ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyp OCA], [https://pdbe.org/2uyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uyp RCSB], [https://www.ebi.ac.uk/pdbsum/2uyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TDCF_ECOLI TDCF_ECOLI]] May be a post-translational regulator that controls the metabolic fate of L-threonine or the potentially toxic intermediate 2-ketobutyrate.
| + | [https://www.uniprot.org/uniprot/TDCF_ECOLI TDCF_ECOLI] May be a post-translational regulator that controls the metabolic fate of L-threonine or the potentially toxic intermediate 2-ketobutyrate. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Burman, J D]] | + | [[Category: Burman JD]] |
| - | [[Category: Lawson, D M]] | + | [[Category: Lawson DM]] |
| - | [[Category: Sawers, R G]] | + | [[Category: Sawers RG]] |
| - | [[Category: Stevenson, C E.M]] | + | [[Category: Stevenson CEM]] |
| - | [[Category: 2-ketobutyrate]]
| + | |
| - | [[Category: Ligand binding]]
| + | |
| - | [[Category: Propionate]]
| + | |
| - | [[Category: Tdcf protein]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| - | [[Category: Yjgf/yer057c/uk114 family]]
| + | |
| Structural highlights
Function
TDCF_ECOLI May be a post-translational regulator that controls the metabolic fate of L-threonine or the potentially toxic intermediate 2-ketobutyrate.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
BACKGROUND: The YjgF/YER057c/UK114 family of proteins is widespread in nature, but has as yet no clearly defined biological role. Members of the family exist as homotrimers and are characterised by intersubunit clefts that are delineated by well-conserved residues; these sites are likely to be of functional significance, yet catalytic activity has never been detected for any member of this family. The gene encoding the TdcF protein of E. coli, a YjgF/YER057c/UK114 family member, resides in an operon that strongly suggests a role in the metabolism of 2-ketobutyrate for this protein. RESULTS: We have determined the crystal structure of E. coli TdcF by molecular replacement to a maximum resolution of 1.6 A. Structures are also presented of TdcF complexed with a variety of ligands. CONCLUSION: The TdcF structure closely resembles those of all YjgF/YER057c/UK114 family members determined thus far. It has the trimeric quaternary structure and intersubunit cavities characteristic of this family of proteins. We show that TdcF is capable of binding several low molecular weight metabolites bearing a carboxylate group, although the interaction with 2-ketobutyrate appears to be the most well defined. These observations may be indicative of a role for TdcF in sensing this potentially toxic metabolite.
The crystal structure of Escherichia coli TdcF, a member of the highly conserved YjgF/YER057c/UK114 family.,Burman JD, Stevenson CE, Sawers RG, Lawson DM BMC Struct Biol. 2007 May 16;7:30. PMID:17506874[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Burman JD, Stevenson CE, Sawers RG, Lawson DM. The crystal structure of Escherichia coli TdcF, a member of the highly conserved YjgF/YER057c/UK114 family. BMC Struct Biol. 2007 May 16;7:30. PMID:17506874 doi:10.1186/1472-6807-7-30
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