7s4m
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution== |
| - | <StructureSection load='7s4m' size='340' side='right'caption='[[7s4m]]' scene=''> | + | <StructureSection load='7s4m' size='340' side='right'caption='[[7s4m]], [[Resolution|resolution]] 2.42Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7s4m]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylocystis_sp._ATCC_49242 Methylocystis sp. ATCC 49242]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S4M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s4m OCA], [https://pdbe.org/7s4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s4m RCSB], [https://www.ebi.ac.uk/pdbsum/7s4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s4m ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.42Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HXG:1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>HXG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s4m OCA], [https://pdbe.org/7s4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s4m RCSB], [https://www.ebi.ac.uk/pdbsum/7s4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s4m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C0LJI4_9HYPH C0LJI4_9HYPH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function. | ||
| + | |||
| + | Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.,Koo CW, Tucci FJ, He Y, Rosenzweig AC Science. 2022 Mar 18;375(6586):1287-1291. doi: 10.1126/science.abm3282. Epub 2022 , Mar 17. PMID:35298269<ref>PMID:35298269</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7s4m" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Methylocystis sp. ATCC 49242]] |
| + | [[Category: Koo CW]] | ||
| + | [[Category: Rosenzweig AC]] | ||
Current revision
CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution
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