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7mqq

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Current revision (07:19, 3 April 2024) (edit) (undo)
 
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<StructureSection load='7mqq' size='340' side='right'caption='[[7mqq]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
<StructureSection load='7mqq' size='340' side='right'caption='[[7mqq]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7mqq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MQQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7mqq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Puccinia_graminis_f._sp._tritici Puccinia graminis f. sp. tritici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MQQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mqq OCA], [https://pdbe.org/7mqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mqq RCSB], [https://www.ebi.ac.uk/pdbsum/7mqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mqq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mqq OCA], [https://pdbe.org/7mqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mqq RCSB], [https://www.ebi.ac.uk/pdbsum/7mqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mqq ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A5B0MRS6_PUCGR A0A5B0MRS6_PUCGR]
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Pathogen effectors are crucial players during plant colonization and infection. Plant resistance mostly relies on effector recognition to activate defence responses. Understanding how effector proteins escape from plant surveillance is important for plant breeding and resistance deployment. Here we examined the role of genetic diversity of the stem rust (Puccinia graminis f. sp. tritici (Pgt)) AvrSr50 gene in determining recognition by the corresponding wheat Sr50 resistance gene. We solved the crystal structure of a natural variant of AvrSr50 and used site-directed mutagenesis and transient expression assays to dissect the molecular mechanisms explaining gain of virulence. We report that AvrSr50 can escape recognition by Sr50 through different mechanisms including DNA insertion, stop codon loss, or by amino acid variation involving a single substitution of the AvrSr50 surface exposed residue Q121. We also report structural homology of AvrSr50 to cupin superfamily members and carbohydrate binding modules indicating a potential role in binding sugar moieties. This study identifies key polymorphic sites present in AvrSr50 alleles from natural stem rust populations that play important roles to escape from Sr50 recognition. This constitutes an important step to better understand Pgt effector evolution and to monitor AvrSr50 variants in natural rust populations.
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The stem rust effector protein AvrSr50 escapes Sr50 recognition by a substitution in a single surface exposed residue.,Ortiz D, Chen J, Outram MA, Saur IML, Upadhyaya NM, Mago R, Ericsson DJ, Cesari S, Chen C, Williams SJ, Dodds PN New Phytol. 2022 Feb 2. doi: 10.1111/nph.18011. PMID:35107838<ref>PMID:35107838</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7mqq" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ericsson, D J]]
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[[Category: Puccinia graminis f. sp. tritici]]
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[[Category: Outram, M A]]
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[[Category: Ericsson DJ]]
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[[Category: Williams, S J]]
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[[Category: Outram MA]]
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[[Category: Effector]]
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[[Category: Williams SJ]]
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[[Category: Stem rust]]
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[[Category: Unknown function]]
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Current revision

Structure of an allelic variant of Puccinia graminis f. sp. tritici (Pgt) effector AvrSr50 (QCMJC)

PDB ID 7mqq

Drag the structure with the mouse to rotate

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