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| | <StructureSection load='2vs4' size='340' side='right'caption='[[2vs4]], [[Resolution|resolution]] 1.77Å' scene=''> | | <StructureSection load='2vs4' size='340' side='right'caption='[[2vs4]], [[Resolution|resolution]] 1.77Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vs4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VS4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vs4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VS4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1vzt|1vzt]], [[2jcf|2jcf]], [[1gx0|1gx0]], [[1vzx|1vzx]], [[1o7q|1o7q]], [[1gx4|1gx4]], [[2jcj|2jcj]], [[2jck|2jck]], [[2vfz|2vfz]], [[1g93|1g93]], [[1g8o|1g8o]], [[1gwv|1gwv]], [[1k4v|1k4v]], [[1vzu|1vzu]], [[1o7o|1o7o]], [[2jcl|2jcl]], [[2jco|2jco]], [[1gww|1gww]], [[1fg5|1fg5]], [[2vs3|2vs3]], [[2vs5|2vs5]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PRD_900019:N-acetyl-alpha-lactosamine'>PRD_900019</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vs4 OCA], [https://pdbe.org/2vs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vs4 RCSB], [https://www.ebi.ac.uk/pdbsum/2vs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vs4 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vs4 OCA], [https://pdbe.org/2vs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vs4 RCSB], [https://www.ebi.ac.uk/pdbsum/2vs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vs4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
| + | [https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bovin]] | + | [[Category: Bos taurus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
| + | [[Category: Acharya KR]] |
| - | [[Category: Acharya, K R]] | + | [[Category: Brew K]] |
| - | [[Category: Brew, K]] | + | [[Category: Jamaluddin H]] |
| - | [[Category: Jamaluddin, H]] | + | [[Category: Thiyagarajan N]] |
| - | [[Category: Thiyagarajan, N]] | + | [[Category: Tumbale P]] |
| - | [[Category: Tumbale, P]] | + | |
| - | [[Category: Active-site mutant]]
| + | |
| - | [[Category: Alpha-3gt]]
| + | |
| - | [[Category: Alpha3gt]]
| + | |
| - | [[Category: Galactose]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Golgi apparatus]]
| + | |
| - | [[Category: Gt]]
| + | |
| - | [[Category: Manganese]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: N-acetyl lactosamine]]
| + | |
| - | [[Category: Signal-anchor]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Udp]]
| + | |
| Structural highlights
2vs4 is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.77Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
GGTA1_BOVIN Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
alpha-1,3-Galactosyltransferase (alpha3GT) catalyzes the transfer of galactose from UDP-galactose to form an alpha 1-3 link with beta-linked galactosides; it is part of a family of homologous retaining glycosyltransferases that includes the histo-blood group A and B glycosyltransferases, Forssman glycolipid synthase, iGb3 synthase, and some uncharacterized prokaryotic glycosyltransferases. In mammals, the presence or absence of active forms of these enzymes results in antigenic differences between individuals and species that modulate the interplay between the immune system and pathogens. The catalytic mechanism of alpha3GT is controversial, but the structure of an enzyme complex with the donor substrate could illuminate both this and the basis of donor substrate specificity. We report here the structure of the complex of a low-activity mutant alpha3GT with UDP-galactose (UDP-gal) exhibiting a bent configuration stabilized by interactions of the galactose with multiple residues in the enzyme including those in a highly conserved region (His315 to Ser318). Analysis of the properties of mutants containing substitutions for these residues shows that catalytic activity is strongly affected by His315 and Asp316. The negative charge of Asp316 is crucial for catalytic activity, and structural studies of two mutants show that its interaction with Arg202 is needed for an active site structure that facilitates the binding of UDP-gal in a catalytically competent conformation.
Structural Basis of UDP-galactose Binding by alpha-1,3-Galactosyltransferase (alpha3GT): Role of Negative Charge on Aspartic Acid 316 in Structure and Activity.,Tumbale P, Jamaluddin H, Thiyagarajan N, Brew K, Acharya KR Biochemistry. 2008 Jul 24. PMID:18651752[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tumbale P, Jamaluddin H, Thiyagarajan N, Brew K, Acharya KR. Structural Basis of UDP-galactose Binding by alpha-1,3-Galactosyltransferase (alpha3GT): Role of Negative Charge on Aspartic Acid 316 in Structure and Activity. Biochemistry. 2008 Jul 24. PMID:18651752 doi:10.1021/bi800852a
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