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| | <StructureSection load='2w2h' size='340' side='right'caption='[[2w2h]], [[Resolution|resolution]] 3.25Å' scene=''> | | <StructureSection load='2w2h' size='340' side='right'caption='[[2w2h]], [[Resolution|resolution]] 3.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Eiav Eiav] and [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus] and [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25Å</td></tr> |
| | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [https://pdbe.org/2w2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [https://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [https://pdbe.org/2w2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [https://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TAT_EIAVY TAT_EIAVY]] Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).
| + | [https://www.uniprot.org/uniprot/CCNT1_HORSE CCNT1_HORSE] Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II.[UniProtKB:O60563] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eiav]] | + | [[Category: Equine infectious anemia virus]] |
| | [[Category: Equus caballus]] | | [[Category: Equus caballus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Anand, K]] | + | [[Category: Anand K]] |
| - | [[Category: Geyer, M]] | + | [[Category: Geyer M]] |
| - | [[Category: Acetylation]]
| + | |
| - | [[Category: Activator]]
| + | |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Cell division]]
| + | |
| - | [[Category: Coiled coil]]
| + | |
| - | [[Category: Crystallization]]
| + | |
| - | [[Category: Cyclin]]
| + | |
| - | [[Category: Cyclin t1]]
| + | |
| - | [[Category: Host-virus interaction]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Rna-binding protein]]
| + | |
| - | [[Category: Tar]]
| + | |
| - | [[Category: Tat]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Function
CCNT1_HORSE Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II.[UniProtKB:O60563]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The replication of many retroviruses is mediated by a transcriptional activator protein, Tat, which activates RNA polymerase II at the level of transcription elongation. Tat interacts with Cyclin T1 of the positive transcription-elongation factor P-TEFb to recruit the transactivation-response TAR RNA, which acts as a promoter element in the transcribed 5' end of the viral long terminal repeat. Here we present the structure of the cyclin box domain of Cyclin T1 in complex with the Tat protein from the equine infectious anemia virus and its corresponding TAR RNA. The basic RNA-recognition motif of Tat adopts a helical structure whose flanking regions interact with a cyclin T-specific loop in the first cyclin box repeat. Together, both proteins coordinate the stem-loop structure of TAR. Our findings show that Tat binds to a surface on Cyclin T1 similar to where recognition motifs from substrate and inhibitor peptides were previously found to interact within Cdk-cyclin pairs.
Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.,Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M Nat Struct Mol Biol. 2008 Dec;15(12):1287-92. Epub 2008 Nov 23. PMID:19029897[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M. Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV. Nat Struct Mol Biol. 2008 Dec;15(12):1287-92. Epub 2008 Nov 23. PMID:19029897 doi:10.1038/nsmb.1513
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