7ei0
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of falcipain 2 from 3D7 strain== | ==Crystal structure of falcipain 2 from 3D7 strain== | ||
| - | <StructureSection load='7ei0' size='340' side='right'caption='[[7ei0]]' scene=''> | + | <StructureSection load='7ei0' size='340' side='right'caption='[[7ei0]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EI0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ei0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EI0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ei0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ei0 OCA], [https://pdbe.org/7ei0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ei0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ei0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ei0 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=YCM:S-(2-AMINO-2-OXOETHYL)-L-CYSTEINE'>YCM</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ei0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ei0 OCA], [https://pdbe.org/7ei0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ei0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ei0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ei0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8I6U4_PLAF7 Q8I6U4_PLAF7] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Malaria identifies as a tropical hallmark, conforming to the burgeoning notion of escalating drug resistance among virulent strains, with the burdensome Plasmodium falciparum under its wing. The cysteine protease Falcipain-2 (FP2) is released in the parasite's food vacuole in the trophozoite stage and contributes to disease progression through its hemoglobinase activity. In the present study, we have determined the crystal structure of FP2 from a drug resistant P. falciparum 3D7 strain. FP2-3D7 sequence has detected four amino acid variants, R12K, E14 N, P100T and G102D, in the mature domain of the protease, when compared with other reported structures. FP2-3D7 protease has been crystallized in the presence of two inhibitors E-64 and Iodoacetamide, which diffracted up to 3.5 A and 3.4 A respectively. Structural analyses of the mature domain helped unveil two solvent-exposed pockets with bound ligands where one is structurally homologous to the allosteric binding site of human Cathepsin-K and thus, could be exploited for designing allosteric modifiers of FP2. The structure has also revealed (poly)ethylene glycol molecules along the catalytic cleft, providing interesting insights for exploring FP2 as a chemotherapeutic target and for PEGylation in drug delivery. The side-chains of P2 and P3 residues of E-64 also adopt different rotamer conformations, compared with previously reported structure, emphasizing strain-specific multiple binding-modes of active-site targeted inhibitors. Docking studies, along with normal mode analyses, highlight the mode of hemoglobin binding and the active/inactive switch in hemoglobinase activity, conjecturing the formation of a stable dimeric state with a symmetry related copy in crystal packing. | ||
| + | |||
| + | New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain.,Chakraborty S, Alam B, Biswas S Biochem Biophys Res Commun. 2022 Jan 29;590:145-151. doi:, 10.1016/j.bbrc.2021.12.080. Epub 2021 Dec 23. PMID:34974303<ref>PMID:34974303</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ei0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Plasmodium falciparum 3D7]] | ||
[[Category: Biswas S]] | [[Category: Biswas S]] | ||
[[Category: Chakraborty S]] | [[Category: Chakraborty S]] | ||
Revision as of 19:29, 19 October 2022
Crystal structure of falcipain 2 from 3D7 strain
| |||||||||||
