This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7qbp
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea== | ==Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea== | ||
| - | <StructureSection load='7qbp' size='340' side='right'caption='[[7qbp]]' scene=''> | + | <StructureSection load='7qbp' size='340' side='right'caption='[[7qbp]], [[Resolution|resolution]] 1.38Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qbp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbp OCA], [https://pdbe.org/7qbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbp RCSB], [https://www.ebi.ac.uk/pdbsum/7qbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbp ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7qbk|7qbk]]</div></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbp OCA], [https://pdbe.org/7qbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbp RCSB], [https://www.ebi.ac.uk/pdbsum/7qbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/RIR2H_SACEN RIR2H_SACEN]] Probable oxidase. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | R2-like ligand-binding oxidase (R2lox) is a ferritin-like protein that harbours a heterodinuclear manganese-iron active site. Although R2lox function is yet to be established, the enzyme binds a fatty acid ligand coordinating the metal centre and catalyses the formation of a tyrosine-valine ether cross-link in the protein scaffold upon O2 activation. Here, we characterized the ligands copurified with R2lox by mass spectrometry-based metabolomics. Moreover, we present the crystal structures of two new homologs of R2lox, from Saccharopolyspora erythraea and Sulfolobus acidocaldarius, at 1.38 A and 2.26 A resolution, respectively, providing the highest resolution structure for R2lox, as well as new insights into putative mechanisms regulating the function of the enzyme. | ||
| + | |||
| + | Comparative structural analysis provides new insights into the function of R2-like ligand-binding oxidase.,Diamanti R, Srinivas V, Johansson AI, Nordstrom A, Griese JJ, Lebrette H, Hogbom M FEBS Lett. 2022 Feb 17. doi: 10.1002/1873-3468.14319. PMID:35175627<ref>PMID:35175627</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qbp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Diamanti R]] | + | [[Category: Diamanti, R]] |
| - | [[Category: Hogbom M]] | + | [[Category: Hogbom, M]] |
| - | [[Category: Lebrette H]] | + | [[Category: Lebrette, H]] |
| - | [[Category: Srinivas V]] | + | [[Category: Srinivas, V]] |
| + | [[Category: Ferritin-like superfamily]] | ||
| + | [[Category: Metalloprotein]] | ||
| + | [[Category: Mn/fe cofactor]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: R2-like ligand-binding oxidase]] | ||
| + | [[Category: R2lox]] | ||
| + | [[Category: Ribonucleotide reductase r2 subunit fold]] | ||
Revision as of 16:23, 6 July 2022
Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea
| |||||||||||
