7w5z
From Proteopedia
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| - | ==== | + | ==Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model== |
| - | <StructureSection load='7w5z' size='340' side='right'caption='[[7w5z]]' scene=''> | + | <StructureSection load='7w5z' size='340' side='right'caption='[[7w5z]], [[Resolution|resolution]] 3.02Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7w5z]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W5Z FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w5z OCA], [https://pdbe.org/7w5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w5z RCSB], [https://www.ebi.ac.uk/pdbsum/7w5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w5z ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.02Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w5z OCA], [https://pdbe.org/7w5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w5z RCSB], [https://www.ebi.ac.uk/pdbsum/7w5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w5z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q22C06_TETTS Q22C06_TETTS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Respiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and structural diversity of eukaryotic respiration, we examined the respiratory chain of the ciliate Tetrahymena thermophila (Tt). Using cryo-electron microscopy on a mixed sample, we solved structures of a supercomplex between Tt complex I (Tt-CI) and Tt-CIII(2) (Tt-SC I+III(2)) and a structure of Tt-CIV(2). Tt-SC I+III(2) (~2.3 megadaltons) is a curved assembly with structural and functional symmetry breaking. Tt-CIV(2) is a ~2.7-megadalton dimer with more than 50 subunits per protomer, including mitochondrial carriers and a TIM8(3)-TIM13(3)-like domain. Our structural and functional study of the T. thermophila respiratory chain reveals divergence in key components of eukaryotic respiration, thereby expanding our understanding of core metabolism. | ||
| + | |||
| + | Structures of Tetrahymena's respiratory chain reveal the diversity of eukaryotic core metabolism.,Zhou L, Maldonado M, Padavannil A, Guo F, Letts JA Science. 2022 May 20;376(6595):831-839. doi: 10.1126/science.abn7747. Epub 2022 , Mar 31. PMID:35357889<ref>PMID:35357889</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7w5z" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cytochrome c oxidase 3D structures|Cytochrome c oxidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Tetrahymena thermophila]] |
| + | [[Category: Letts J]] | ||
| + | [[Category: Maldonado M]] | ||
| + | [[Category: Padavannil A]] | ||
| + | [[Category: Zhou L]] | ||
Current revision
Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model
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