2wfm

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of polyneuridine aldehyde esterase mutant (H244A)

Structural highlights

2wfm is a 5 chain structure with sequence from Rauvolfia serpentina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PNAE_RAUSE Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cutting carbons: The three-dimensional structure of polyneuridine aldehyde esterase (PNAE) gives insight into the enzymatic mechanism of the biosynthesis of C(9)- from C(10)-monoterpenoid indole alkaloids (see scheme). PNAE is a very substrate-specific serine esterase. It harbors the catalytic triad S87-D216-H244, and is a new member of the alpha/beta-fold hydrolase superfamily. Its novel function leads to the diversification of alkaloid structures.

Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids.,Yang L, Hill M, Wang M, Panjikar S, Stockigt J Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family. Eur J Biochem. 2000 Mar;267(5):1397-406. PMID:10691977
↑ Yang L, Hill M, Wang M, Panjikar S, Stockigt J. Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids. Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101 doi:10.1002/anie.200900150

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wfm"

@@ Line 3: / Line 3: @@
 <StructureSection load='2wfm' size='340' side='right'caption='[[2wfm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wfm]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WFM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wfm]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WFM FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wfl|2wfl]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Polyneuridine-aldehyde_esterase Polyneuridine-aldehyde esterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.78 3.1.1.78] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfm OCA], [https://pdbe.org/2wfm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wfm RCSB], [https://www.ebi.ac.uk/pdbsum/2wfm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfm ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE]] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref>
+[https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 37: / Line 36: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Ophioxylon serpentinum]]
+[[Category: Rauvolfia serpentina]]
-[[Category: Polyneuridine-aldehyde esterase]]
+[[Category: Hill M]]
-[[Category: Hill, M]]
+[[Category: Panjikar S]]
-[[Category: Panjikar, S]]
+[[Category: Stoeckigt J]]
-[[Category: Stoeckigt, J]]
+[[Category: Wang M]]
-[[Category: Wang, M]]
+[[Category: Yang L]]
-[[Category: Yang, L]]
-[[Category: Alkaloid metabolism]]
-[[Category: Hydrolase]]
-[[Category: Monoterpenoid indole alkaloid]]
-[[Category: Pnae]]
-[[Category: Serine esterase]]

2wfm

From Proteopedia

Current revision

Crystal structure of polyneuridine aldehyde esterase mutant (H244A)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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