1ebp
From Proteopedia
(New page: 200px<br /> <applet load="1ebp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebp, resolution 2.8Å" /> '''COMPLEX BETWEEN THE ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ebp.gif|left|200px]]<br /> | + | [[Image:1ebp.gif|left|200px]]<br /><applet load="1ebp" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ebp" size=" | + | |
caption="1ebp, resolution 2.8Å" /> | caption="1ebp, resolution 2.8Å" /> | ||
'''COMPLEX BETWEEN THE EXTRACELLULAR DOMAIN OF ERYTHROPOIETIN (EPO) RECEPTOR [EBP] AND AN AGONIST PEPTIDE [EMP1]'''<br /> | '''COMPLEX BETWEEN THE EXTRACELLULAR DOMAIN OF ERYTHROPOIETIN (EPO) RECEPTOR [EBP] AND AN AGONIST PEPTIDE [EMP1]'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The functional mimicry of a protein by an unrelated small molecule has | + | The functional mimicry of a protein by an unrelated small molecule has been a formidable challenge. Now, however, the biological activity of a 166-residue hematopoietic growth hormone, erythropoietin (EPO), with its class 1 cytokine receptor has been mimicked by a 20-residue cyclic peptide unrelated in sequence to the natural ligand. The crystal structure at 2.8 A resolution of a complex of this agonist peptide with the extracellular domain of EPO receptor reveals that a peptide dimer induces an almost perfect twofold dimerization of the receptor. The dimer assembly differs from that of the human growth hormone (hGH) receptor complex and suggests that more than one mode of dimerization may be able to induce signal transduction and cell proliferation. The EPO receptor binding site, defined by peptide interaction, corresponds to the smaller functional epitope identified for hGH receptor. Similarly, the EPO mimetic peptide ligand can be considered as a minimal hormone, and suggests the design of nonpeptidic small molecule mimetics for EPO and other cytokines may indeed be achievable. |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1EBP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1EBP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBP OCA]. |
==Reference== | ==Reference== | ||
| Line 18: | Line 17: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Livnah, O.]] | [[Category: Livnah, O.]] | ||
| - | [[Category: Stura, E | + | [[Category: Stura, E A.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: complex (cytokine receptor/peptide)]] | [[Category: complex (cytokine receptor/peptide)]] | ||
[[Category: cytokine receptor class 1]] | [[Category: cytokine receptor class 1]] | ||
| Line 27: | Line 26: | ||
[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:02 2008'' |
Revision as of 10:26, 21 February 2008
|
COMPLEX BETWEEN THE EXTRACELLULAR DOMAIN OF ERYTHROPOIETIN (EPO) RECEPTOR [EBP] AND AN AGONIST PEPTIDE [EMP1]
Contents |
Overview
The functional mimicry of a protein by an unrelated small molecule has been a formidable challenge. Now, however, the biological activity of a 166-residue hematopoietic growth hormone, erythropoietin (EPO), with its class 1 cytokine receptor has been mimicked by a 20-residue cyclic peptide unrelated in sequence to the natural ligand. The crystal structure at 2.8 A resolution of a complex of this agonist peptide with the extracellular domain of EPO receptor reveals that a peptide dimer induces an almost perfect twofold dimerization of the receptor. The dimer assembly differs from that of the human growth hormone (hGH) receptor complex and suggests that more than one mode of dimerization may be able to induce signal transduction and cell proliferation. The EPO receptor binding site, defined by peptide interaction, corresponds to the smaller functional epitope identified for hGH receptor. Similarly, the EPO mimetic peptide ligand can be considered as a minimal hormone, and suggests the design of nonpeptidic small molecule mimetics for EPO and other cytokines may indeed be achievable.
Disease
Known disease associated with this structure: Erythrocytosis, familial OMIM:[133171]
About this Structure
1EBP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Functional mimicry of a protein hormone by a peptide agonist: the EPO receptor complex at 2.8 A., Livnah O, Stura EA, Johnson DL, Middleton SA, Mulcahy LS, Wrighton NC, Dower WJ, Jolliffe LK, Wilson IA, Science. 1996 Jul 26;273(5274):464-71. PMID:8662530
Page seeded by OCA on Thu Feb 21 12:26:02 2008
