1edm
From Proteopedia
(New page: 200px<br /> <applet load="1edm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1edm, resolution 1.5Å" /> '''EPIDERMAL GROWTH FAC...) |
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| - | [[Image:1edm.gif|left|200px]]<br /> | + | [[Image:1edm.gif|left|200px]]<br /><applet load="1edm" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1edm" size=" | + | |
caption="1edm, resolution 1.5Å" /> | caption="1edm, resolution 1.5Å" /> | ||
'''EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX'''<br /> | '''EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Various diverse extracellular proteins possess Ca(2+)-binding epidermal | + | Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1EDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brownlee, G | + | [[Category: Brownlee, G G.]] |
[[Category: Handford, P.]] | [[Category: Handford, P.]] | ||
[[Category: Knott, V.]] | [[Category: Knott, V.]] | ||
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[[Category: structure and function]] | [[Category: structure and function]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:36 2008'' |
Revision as of 10:26, 21 February 2008
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EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX
Contents |
Overview
Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains.
Disease
Known diseases associated with this structure: Hemophilia B OMIM:[306900], Warfarin sensitivity OMIM:[306900]
About this Structure
1EDM is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions., Rao Z, Handford P, Mayhew M, Knott V, Brownlee GG, Stuart D, Cell. 1995 Jul 14;82(1):131-41. PMID:7606779
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