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1gua

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[[Image:1gua.gif|left|200px]]
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{{STRUCTURE_1gua| PDB=1gua | SCENE= }}
{{STRUCTURE_1gua| PDB=1gua | SCENE= }}
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'''HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131'''
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===HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131===
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==Overview==
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Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF). To address the question of specificity of interactions we have introduced the mutations E30D and K31E into Rap and show biochemically, by X-ray structure analysis and by transfection in vivo that the identical core effector region of Ras and Rap (residues 32-40) is responsible for molecular recognition, but that residues outside this region are responsible for the specificity of the interaction. The major determinant for the switch in specificity is the opposite charge of residue 31--Lys in Rap, Glu in Ras--which creates a favourable complementary interface for the Ras-Raf interaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 8756332 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8756332}}
==About this Structure==
==About this Structure==
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[[Category: Wittinghofer, A.]]
[[Category: Wittinghofer, A.]]
[[Category: Oncogene protein/kinase/effector protein gtp-binding-protein]]
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Revision as of 03:05, 1 July 2008

Image:1gua.png

Template:STRUCTURE 1gua

HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131

Template:ABSTRACT PUBMED 8756332

About this Structure

1GUA is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Ras/Rap effector specificity determined by charge reversal., Nassar N, Horn G, Herrmann C, Block C, Janknecht R, Wittinghofer A, Nat Struct Biol. 1996 Aug;3(8):723-9. PMID:8756332

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