1gur

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{{STRUCTURE_1gur| PDB=1gur | SCENE= }}
{{STRUCTURE_1gur| PDB=1gur | SCENE= }}
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'''GURMARIN, A SWEET TASTE-SUPPRESSING POLYPEPTIDE, NMR, 10 STRUCTURES'''
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===GURMARIN, A SWEET TASTE-SUPPRESSING POLYPEPTIDE, NMR, 10 STRUCTURES===
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==Overview==
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The solution structure of gurmarin was studied by two-dimensional proton NMR spectroscopy at 600 MHz. Gurmarin, a 35-amino acid residue polypeptide recently discovered in an Indian-originated tree Gymnema sylvestre, selectively suppresses the neural responses of rat to sweet taste stimuli. Sequence-specific resonance assignments were obtained for all backbone protons and for most of the side-chain protons. The three-dimensional solution structure was determined by simulated-annealing calculations on the basis of 135 interproton distance constraints derived from NOEs, six distance constraints for three hydrogen bonds and 16 dihedral angle constraints derived from coupling constants. A total of 10 structures folded into a well-defined structure with a triple-stranded antiparallel beta-sheet. The average rmsd values between any two structures were 1.65 +/- 0.39 A for the backbone atoms (N, C alpha, C) and 2.95 +/- 0.27 A for all heavy atoms. The positions of the three disulfide bridges, which could not be determined chemically, were estimated to be Cys3-Cys18, Cys10-Cys23 and Cys17-Cys33 on the basis of the NMR distance constraints. This disulfide bridge pattern in gurmarin turned out to be analogous to that in omega-conotoxin and Momordica charantia trypsin inhibitor-II, and the topology of folding was the same as that in omega-conotoxin.
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{{ABSTRACT_PUBMED_7787425}}
==About this Structure==
==About this Structure==
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1GUR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gymnema_sylvestre Gymnema sylvestre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUR OCA].
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1GUR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gymnema_sylvestre Gymnema sylvestre]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUR OCA].
==Reference==
==Reference==
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[[Category: Suppressing protein]]
[[Category: Suppressing protein]]
[[Category: Sweet-taste]]
[[Category: Sweet-taste]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:02:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:06:57 2008''

Revision as of 03:06, 1 July 2008

Image:1gur.png

Template:STRUCTURE 1gur

GURMARIN, A SWEET TASTE-SUPPRESSING POLYPEPTIDE, NMR, 10 STRUCTURES

Template:ABSTRACT PUBMED 7787425

About this Structure

1GUR is a Single protein structure of sequence from Gymnema sylvestre. Full experimental information is available from OCA.

Reference

Three-dimensional structure of gurmarin, a sweet taste-suppressing polypeptide., Arai K, Ishima R, Morikawa S, Miyasaka A, Imoto T, Yoshimura S, Aimoto S, Akasaka K, J Biomol NMR. 1995 Apr;5(3):297-305. PMID:7787425

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