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| - | | + | #REDIRECT [[5vph]] This PDB entry is obsolete and replaced by 5vph |
| - | ==Crystal structure of Der p 1 complexed with Fab 4C1==
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| - | <StructureSection load='3rvx' size='340' side='right'caption='[[3rvx]], [[Resolution|resolution]] 2.50Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3rvx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dermatophagoides_pteronyssinus Dermatophagoides pteronyssinus] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RVX FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rvt|3rvt]], [[3rvu|3rvu]], [[3rvv|3rvv]], [[3rvw|3rvw]]</div></td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidase_1_(mite) Peptidase 1 (mite)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.65 3.4.22.65] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rvx OCA], [https://pdbe.org/3rvx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rvx RCSB], [https://www.ebi.ac.uk/pdbsum/3rvx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rvx ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[https://www.uniprot.org/uniprot/PEPT1_DERPT PEPT1_DERPT]] Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | House dust mites produce potent allergens, Der p 1 and Der f 1, that cause allergic sensitization and asthma. Der p 1 and Der f 1 are cysteine proteases that elicit IgE responses in 80% of mite-allergic subjects and have proinflammatory properties. Their antigenic structure is unknown. Here, we present crystal structures of natural Der p 1 and Der f 1 in complex with a monoclonal antibody, 4C1, which binds to a unique cross-reactive epitope on both allergens associated with IgE recognition. The 4C1 epitope is formed by almost identical amino acid sequences and contact residues. Mutations of the contact residues abrogate mAb 4C1 binding and reduce IgE antibody binding. These surface-exposed residues are molecular targets that can be exploited for development of recombinant allergen vaccines.
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| - | Molecular determinants for antibody binding on group 1 house dust mite allergens.,Chruszcz M, Pomes A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TA, Minor W, Chapman MD J Biol Chem. 2012 Mar 2;287(10):7388-98. Epub 2011 Dec 30. PMID:22210776<ref>PMID:22210776</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3rvx" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Dermatophagoides pteronyssinus]]
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| - | [[Category: Large Structures]]
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| - | [[Category: Lk3 transgenic mice]]
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| - | [[Category: Chapman, M D]]
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| - | [[Category: Chruszcz, M]]
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| - | [[Category: Minor, W]]
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| - | [[Category: Pomes, A]]
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| - | [[Category: Vailes, L D]]
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| - | [[Category: Allergen-antibody complex]]
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| - | [[Category: Der p 1]]
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| - | [[Category: Hydrolase-immune system complex]]
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