1efn
From Proteopedia
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| - | [[Image:1efn.gif|left|200px]]<br /> | + | [[Image:1efn.gif|left|200px]]<br /><applet load="1efn" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1efn" size=" | + | |
caption="1efn, resolution 2.5Å" /> | caption="1efn, resolution 2.5Å" /> | ||
'''HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN'''<br /> | '''HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the conserved core of HIV-1 Nef has been | + | The crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly. The conserved PxxP sequence motif of Nef, known to be important for optimal viral replication, is part of a polyproline type II helix that engages the SH3 domain in a manner resembling closely the interaction of isolated peptides with SH3 domains. The Nef-SH3 structure also reveals how high affinity and specificity in the SH3 interaction is achieved by the presentation of the PxxP motif within the context of the folded structure of Nef. |
==About this Structure== | ==About this Structure== | ||
| - | 1EFN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with PBM as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http:// | + | 1EFN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with <scene name='pdbligand=PBM:'>PBM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Transferase]] | [[Category: Transferase]] | ||
[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
| - | [[Category: Lee, C | + | [[Category: Lee, C H.]] |
[[Category: PBM]] | [[Category: PBM]] | ||
[[Category: aids]] | [[Category: aids]] | ||
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[[Category: tyrosine-protein kinase]] | [[Category: tyrosine-protein kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:14 2008'' |
Revision as of 10:27, 21 February 2008
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HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN
Overview
The crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly. The conserved PxxP sequence motif of Nef, known to be important for optimal viral replication, is part of a polyproline type II helix that engages the SH3 domain in a manner resembling closely the interaction of isolated peptides with SH3 domains. The Nef-SH3 structure also reveals how high affinity and specificity in the SH3 interaction is achieved by the presentation of the PxxP motif within the context of the folded structure of Nef.
About this Structure
1EFN is a Protein complex structure of sequences from Homo sapiens and Human immunodeficiency virus 1 with as ligand. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.
Reference
Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:8681387
Page seeded by OCA on Thu Feb 21 12:27:14 2008
Categories: Homo sapiens | Human immunodeficiency virus 1 | Protein complex | Transferase | Kuriyan, J. | Lee, C H. | PBM | Aids | Atp-binding | Complex (sh3 domain/viral enhancer) | Gtp-binding | Myristylation | Phosphorylation | Ppii helix | Proto-oncogene | Pxxp motif | Sh2 domain | Sh3 domain | Tyrosine-protein kinase
