This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1gvx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1gvx.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1gvx.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1gvx| PDB=1gvx | SCENE= }}
{{STRUCTURE_1gvx| PDB=1gvx | SCENE= }}
-
'''ENDOTHIAPEPSIN COMPLEXED WITH H256'''
+
===ENDOTHIAPEPSIN COMPLEXED WITH H256===
-
==Overview==
+
<!--
-
Endothiapepsin is derived from the fungus Endothia parasitica and is a member of the aspartic proteinase class of enzymes. This class of enzyme is comprised of two structurally similar lobes, each lobe contributing an aspartic acid residue to form a catalytic dyad that acts to cleave the substrate peptide bond. The three-dimensional structures of endothiapepsin bound to five transition state analogue inhibitors (H189, H256, CP-80,794, PD-129,541 and PD-130,328) have been solved at atomic resolution allowing full anisotropic modelling of each complex. The active sites of the five structures have been studied with a view to studying the catalytic mechanism of the aspartic proteinases by locating the active site protons by carboxyl bond length differences and electron density analysis. In the CP-80,794 structure there is excellent electron density for the hydrogen on the inhibitory statine hydroxyl group which forms a hydrogen bond with the inner oxygen of Asp32. The location of this proton has implications for the catalytic mechanism of the aspartic proteinases as it is consistent with the proposed mechanism in which Asp32 is the negatively charged aspartate. A number of short hydrogen bonds (approximately 2.6 A) with ESD values of around 0.01 A that may have a role in catalysis have been identified within the active site of each structure; the lengths of these bonds have been confirmed using NMR techniques. The possibility and implications of low barrier hydrogen bonds in the active site are considered.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12083527}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12083527 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12083527}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Atomic resolution]]
[[Category: Atomic resolution]]
[[Category: Tetrahedral intermediate]]
[[Category: Tetrahedral intermediate]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:04:32 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:11:28 2008''

Revision as of 03:11, 1 July 2008

Image:1gvx.png

Template:STRUCTURE 1gvx

ENDOTHIAPEPSIN COMPLEXED WITH H256

Template:ABSTRACT PUBMED 12083527

About this Structure

1GVX is a Protein complex structure of sequences from Cryphonectria parasitica. Full crystallographic information is available from OCA.

Reference

Five atomic resolution structures of endothiapepsin inhibitor complexes: implications for the aspartic proteinase mechanism., Coates L, Erskine PT, Crump MP, Wood SP, Cooper JB, J Mol Biol. 2002 May 17;318(5):1405-15. PMID:12083527

Page seeded by OCA on Tue Jul 1 06:11:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gvx"
Personal tools