2tdm
From Proteopedia
(New page: 200px<br /> <applet load="2tdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2tdm, resolution 2.55Å" /> '''STRUCTURE OF THYMID...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2TDM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]] with UMP as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1TDM. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TDM OCA]]. | + | 2TDM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]] with UMP as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1TDM. Active as [[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TDM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Lactobacillus casei]] | [[Category: Lactobacillus casei]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Thymidylate synthase]] | ||
[[Category: Finer-Moore, J.]] | [[Category: Finer-Moore, J.]] | ||
[[Category: Stroud, R.M.]] | [[Category: Stroud, R.M.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:34:34 2007'' |
Revision as of 09:29, 30 October 2007
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STRUCTURE OF THYMIDYLATE SYNTHASE
Overview
Crystal structures of two crystal forms of the complex of Lactobacillus, casei (TS) with its substrate dUMP have been solved and refined at 2.55 A, resolution. The two crystal forms differ by approximately 5% in the c-axis, length. The TS-dUMP complexes are symmetric dimers with dUMP bound, equivalently in both active sites. dUMP is non-covalently bound in the, same conformation as in ternary complexes of TS with dUMP and cofactor or, cofactor analogs. The same hydrogen bonds are made between TS and, substrate in the binary and ternary complexes. We have also determined the, 2.36 A crystal structure of phosphate-bound L. casei TS. This structure, has been refined to an R-factor of 19.3% with highly constrained geometry., Refinement has revealed the locations of all residues in the ... [(full description)]
About this Structure
2TDM is a [Single protein] structure of sequence from [Lactobacillus casei] with UMP as [ligand]. This structure superseeds the now removed PDB entry 1TDM. Active as [Thymidylate synthase], with EC number [2.1.1.45]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
Refined structures of substrate-bound and phosphate-bound thymidylate synthase from Lactobacillus casei., Finer-Moore J, Fauman EB, Foster PG, Perry KM, Santi DV, Stroud RM, J Mol Biol. 1993 Aug 20;232(4):1101-16. PMID:8371269
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