7f13
From Proteopedia
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==Crystal structure of isomerase Dcr3== | ==Crystal structure of isomerase Dcr3== | ||
| - | <StructureSection load='7f13' size='340' side='right'caption='[[7f13]]' scene=''> | + | <StructureSection load='7f13' size='340' side='right'caption='[[7f13]], [[Resolution|resolution]] 2.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F13 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7f13]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Diaporthe_longicolla Diaporthe longicolla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F13 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f13 OCA], [https://pdbe.org/7f13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f13 RCSB], [https://www.ebi.ac.uk/pdbsum/7f13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f13 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f13 OCA], [https://pdbe.org/7f13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f13 RCSB], [https://www.ebi.ac.uk/pdbsum/7f13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f13 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The novel isomerase NsrQ, from Aspergillus novofumigatus, is a key enzyme in the biosynthesis of fungal tetrahydroxanthones and is responsible for dearomatizing cyclization to provide a tetrahydroxanthone scaffold. NsrQ catalyzes a two-step isomerization reaction, involving the isomerization of allylic alcohol and subsequent inversion of configuration at the methyl group. We report on the biochemical and structural characterizations of NsrQ, and its homologue Dcr3, from Diaporthe longicolla. The crystal structures of NsrQ and Dcr3 revealed their similar overall structures, with a cone-shaped alpha+beta barrel fold, to those of the nuclear transport factor 2-like superfamily enzymes. Furthermore, the structures of Dcr3 and NsrQ variants complexed with substrate analogues and the site-directed mutagenesis studies identified the catalytic residues and the important hydrophobic residues in shaping the active site pocket for substrate binding. These enzymes thus utilize Glu and His residues as acid-base catalysts. Based on these observations, we proposed a detailed reaction mechanism for NsrQ-catalyzed isomerization reactions. | ||
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| + | Structural Basis for Isomerization Reactions in Fungal Tetrahydroxanthone Biosynthesis and Diversification.,Yang J, Mori T, Wei X, Matsuda Y, Abe I Angew Chem Int Ed Engl. 2021 Aug 23;60(35):19458-19465. doi:, 10.1002/anie.202107884. Epub 2021 Jul 20. PMID:34180120<ref>PMID:34180120</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f13" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Diaporthe longicolla]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Abe I]] | [[Category: Abe I]] | ||
[[Category: Mori T]] | [[Category: Mori T]] | ||
[[Category: Yang J]] | [[Category: Yang J]] | ||
Current revision
Crystal structure of isomerase Dcr3
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