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7vbq
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxIJ== |
| - | <StructureSection load='7vbq' size='340' side='right'caption='[[7vbq]]' scene=''> | + | <StructureSection load='7vbq' size='340' side='right'caption='[[7vbq]], [[Resolution|resolution]] 1.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vbq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_purpureogenus Talaromyces purpureogenus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VBQ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbq OCA], [https://pdbe.org/7vbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbq RCSB], [https://www.ebi.ac.uk/pdbsum/7vbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbq ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbq OCA], [https://pdbe.org/7vbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbq RCSB], [https://www.ebi.ac.uk/pdbsum/7vbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Talaromyolides (1-6) are a group of unusual 6/6/6/6/6/6 hexacyclic meroterpenoids with (3R)-6-hydroxymellein and 4,5-seco-drimane substructures, isolated from the marine fungus Talaromyces purpureogenus. We have identified the biosynthetic gene cluster tlxA-J by heterologous expression in Aspergillus, in vitro enzyme assays, and CRISPR-Cas9-based gene inactivation. Remarkably, the heterodimer of non-heme iron (NHI) enzymes, TlxJ-TlxI, catalyzes three steps of oxidation including a key reaction, hydroxylation at C-5 and C-9 of 12, the intermediate with 3-ketohydroxydrimane scaffold, to facilitate a retro-aldol reaction, leading to the construction of the 4,5-secodrimane skeleton and characteristic ketal scaffold of 1-6. The products of TlxJ-TlxI, 1 and 4, were further hydroxylated at C-4'beta by another NHI heterodimer, TlxA-TlxC, and acetylated by TlxB to yield the final products, 3 and 6. The X-ray structural analysis coupled with site-directed mutagenesis provided insights into the heterodimer TlxJ-TlxI formation and its catalysis. This is the first report to show that two NHI proteins form a heterodimer for catalysis and utilizes a novel methodology to create functional oxygenase structures in secondary metabolite biosynthesis. | ||
| + | |||
| + | Heterodimeric Non-heme Iron Enzymes in Fungal Meroterpenoid Biosynthesis.,Li X, Awakawa T, Mori T, Ling M, Hu D, Wu B, Abe I J Am Chem Soc. 2021 Dec 22;143(50):21425-21432. doi: 10.1021/jacs.1c11548. Epub, 2021 Dec 9. PMID:34881885<ref>PMID:34881885</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vbq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Talaromyces purpureogenus]] |
| + | [[Category: Abe I]] | ||
| + | [[Category: Awakawa T]] | ||
| + | [[Category: Li X]] | ||
| + | [[Category: Mori T]] | ||
Current revision
Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxIJ
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