3j25

From Proteopedia

(Difference between revisions)

Revision as of 14:13, 24 January 2024

Structural basis for TetM-mediated tetracycline resistance

3j25, resolution 7.20Å

Structural highlights

3j25 is a 1 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 7.2Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TET1_ENTFL Abolishes the inhibitory effect of tetracyclin on protein synthesis by a non-covalent modification of the ribosomes.

Publication Abstract from PubMed

Ribosome protection proteins (RPPs) confer tetracycline resistance by binding to the ribosome and chasing the drug from its binding site. The current model for the mechanism of action of RPPs proposes that drug release is indirect and achieved via conformational changes within the drug-binding site induced upon binding of the RPP to the ribosome. Here we report a cryo-EM structure of the RPP TetM in complex with the 70S ribosome at 7.2-A resolution. The structure reveals the contacts of TetM with the ribosome, including interaction between the conserved and functionally critical C-terminal extension of TetM and the decoding center of the small subunit. Moreover, we observe direct interaction between domain IV of TetM and the tetracycline binding site and identify residues critical for conferring tetracycline resistance. A model is presented whereby TetM directly dislodges tetracycline to confer resistance.

Structural basis for TetM-mediated tetracycline resistance.,Donhofer A, Franckenberg S, Wickles S, Berninghausen O, Beckmann R, Wilson DN Proc Natl Acad Sci U S A. 2012 Oct 1. PMID:23027944^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Donhofer A, Franckenberg S, Wickles S, Berninghausen O, Beckmann R, Wilson DN. Structural basis for TetM-mediated tetracycline resistance. Proc Natl Acad Sci U S A. 2012 Oct 1. PMID:23027944 doi:http://dx.doi.org/10.1073/pnas.1208037109

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j25"

@@ Line 3: / Line 3: @@
 <SX load='3j25' size='340' side='right' viewer='molstar' caption='[[3j25]], [[Resolution|resolution]] 7.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J25 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J25 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetM, transposon TnFO1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j25 OCA], [https://pdbe.org/3j25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j25 RCSB], [https://www.ebi.ac.uk/pdbsum/3j25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j25 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/TET1_ENTFL TET1_ENTFL]] Abolishes the inhibitory effect of tetracyclin on protein synthesis by a non-covalent modification of the ribosomes.
+[https://www.uniprot.org/uniprot/TET1_ENTFL TET1_ENTFL] Abolishes the inhibitory effect of tetracyclin on protein synthesis by a non-covalent modification of the ribosomes.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </SX>
-[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
+[[Category: Enterococcus faecalis]]
 [[Category: Large Structures]]
-[[Category: Beckmann, R]]
+[[Category: Beckmann R]]
-[[Category: Berninghausen, O]]
+[[Category: Berninghausen O]]
-[[Category: Doenhoefer, A]]
+[[Category: Doenhoefer A]]
-[[Category: Franckenberg, S]]
+[[Category: Franckenberg S]]
-[[Category: Wickles, S]]
+[[Category: Wickles S]]
-[[Category: Wilson, D N]]
+[[Category: Wilson DN]]
-[[Category: Antibiotic resistance]]
-[[Category: Translation]]

3j25

From Proteopedia

Revision as of 14:13, 24 January 2024

Structural basis for TetM-mediated tetracycline resistance

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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