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| | <SX load='3j2u' size='340' side='right' viewer='molstar' caption='[[3j2u]], [[Resolution|resolution]] 10.80Å' scene=''> | | <SX load='3j2u' size='340' side='right' viewer='molstar' caption='[[3j2u]], [[Resolution|resolution]] 10.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3j2u]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Drome Drome]. The July 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Microtubules'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_7 10.2210/rcsb_pdb/mom_2014_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J2U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3j2u]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The July 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Microtubules'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_7 10.2210/rcsb_pdb/mom_2014_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J2U FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klp10A, CG1453 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.8Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2u OCA], [https://pdbe.org/3j2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j2u RCSB], [https://www.ebi.ac.uk/pdbsum/3j2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2u ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2u OCA], [https://pdbe.org/3j2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j2u RCSB], [https://www.ebi.ac.uk/pdbsum/3j2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/KI10A_DROME KI10A_DROME]] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".<ref>PMID:14681690</ref> [[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
| + | [https://www.uniprot.org/uniprot/KI10A_DROME KI10A_DROME] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".<ref>PMID:14681690</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 27: |
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| | </SX> | | </SX> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Microtubules]] | | [[Category: Microtubules]] |
| | [[Category: RCSB PDB Molecule of the Month]] | | [[Category: RCSB PDB Molecule of the Month]] |
| - | [[Category: Asenjo, A B]] | + | [[Category: Asenjo AB]] |
| - | [[Category: Chatterjee, C]] | + | [[Category: Chatterjee C]] |
| - | [[Category: DePaoli, V]] | + | [[Category: DePaoli V]] |
| - | [[Category: Diaz-Avalos, R]] | + | [[Category: Diaz-Avalos R]] |
| - | [[Category: Rice, W J]] | + | [[Category: Rice WJ]] |
| - | [[Category: Silvestry, M]] | + | [[Category: Silvestry M]] |
| - | [[Category: Sosa, H]] | + | [[Category: Sosa H]] |
| - | [[Category: Tan, D]] | + | [[Category: Tan D]] |
| - | [[Category: Depolymerase]]
| + | |
| - | [[Category: Depolymerization]]
| + | |
| - | [[Category: Kinesin]]
| + | |
| - | [[Category: Kinesin-13]]
| + | |
| - | [[Category: Kinesin13]]
| + | |
| - | [[Category: Kini]]
| + | |
| - | [[Category: Microtubule]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Tubulin]]
| + | |
| Structural highlights
Function
KI10A_DROME Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".[1]
Publication Abstract from PubMed
To elucidate the structural basis of the mechanism of microtubule depolymerization by kinesin-13s, we analyzed complexes of tubulin and the Drosophila melanogaster kinesin-13 KLP10A by electron microscopy (EM) and fluorescence polarization microscopy. We report a nanometer-resolution (1.1 nm) cryo-EM three-dimensional structure of the KLP10A head domain (KLP10AHD) bound to curved tubulin. We found that binding of KLP10AHD induces a distinct tubulin configuration with displacement (shear) between tubulin subunits in addition to curvature. In this configuration, the kinesin-binding site differs from that in straight tubulin, providing an explanation for the distinct interaction modes of kinesin-13s with the microtubule lattice or its ends. The KLP10AHD-tubulin interface comprises three areas of interaction, suggesting a crossbow-type tubulin-bending mechanism. These areas include the kinesin-13 family conserved KVD residues, and as predicted from the crossbow model, mutating these residues changes the orientation and mobility of KLP10AHDs interacting with the microtubule.
Structural Model for Tubulin Recognition and Deformation by Kinesin-13 Microtubule Depolymerases.,Asenjo AB, Chatterjee C, Tan D, Depaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H Cell Rep. 2013 Feb 19. pii: S2211-1247(13)00054-5. doi:, 10.1016/j.celrep.2013.01.030. PMID:23434508[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD, Scholey JM, Sharp DJ. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 2004 Jan 22;427(6972):364-70. Epub 2003 Dec 14. PMID:14681690 doi:10.1038/nature02256
- ↑ Asenjo AB, Chatterjee C, Tan D, Depaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H. Structural Model for Tubulin Recognition and Deformation by Kinesin-13 Microtubule Depolymerases. Cell Rep. 2013 Feb 19. pii: S2211-1247(13)00054-5. doi:, 10.1016/j.celrep.2013.01.030. PMID:23434508 doi:http://dx.doi.org/10.1016/j.celrep.2013.01.030
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