3j6d
From Proteopedia
(Difference between revisions)
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<SX load='3j6d' size='340' side='right' viewer='molstar' caption='[[3j6d]], [[Resolution|resolution]] 11.70Å' scene=''> | <SX load='3j6d' size='340' side='right' viewer='molstar' caption='[[3j6d]], [[Resolution|resolution]] 11.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3j6d]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3j6d]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6D FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.7Å</td></tr> |
| - | + | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6d OCA], [https://pdbe.org/3j6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6d RCSB], [https://www.ebi.ac.uk/pdbsum/3j6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6d ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6d OCA], [https://pdbe.org/3j6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6d RCSB], [https://www.ebi.ac.uk/pdbsum/3j6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/PRGH_SALTY PRGH_SALTY] Required for invasion of epithelial cells. | |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| - | + | The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells of infected organisms. The molecular details of the assembly of this large, multimembrane-spanning complex remain poorly understood. Here, we report structural, biochemical, and functional analyses of PrgK, an inner-membrane component of the prototypical Salmonella typhimurium T3SS. We have obtained the atomic structures of the two ring building globular domains and show that the C-terminal transmembrane helix is not essential for assembly and secretion. We also demonstrate that structural rearrangement of the two PrgK globular domains, driven by an interconnecting linker region, may promote oligomerization into ring structures. Finally, we used electron microscopy-guided symmetry modeling to propose a structural model for the intimately associated PrgH-PrgK ring interaction within the assembled basal body. | |
| - | + | The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body.,Bergeron JR, Worrall LJ, De S, Sgourakis NG, Cheung AH, Lameignere E, Okon M, Wasney GA, Baker D, McIntosh LP, Strynadka NC Structure. 2015 Jan 6;23(1):161-72. doi: 10.1016/j.str.2014.10.021. Epub 2014 Dec, 18. PMID:25533490<ref>PMID:25533490</ref> | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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</SX> | </SX> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Bergeron | + | [[Category: Bergeron JRC]] |
| - | [[Category: Strynadka | + | [[Category: Strynadka NCJ]] |
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Revision as of 14:16, 24 January 2024
Model of the PrgH-PrgK periplasmic rings
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