1gyg

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{{STRUCTURE_1gyg| PDB=1gyg | SCENE= }}
{{STRUCTURE_1gyg| PDB=1gyg | SCENE= }}
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'''R32 CLOSED FORM OF ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS STRAIN CER89L43'''
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===R32 CLOSED FORM OF ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS STRAIN CER89L43===
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==Overview==
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Clostridium perfringens biotype A strains are the causative agents of gas-gangrene in man and are also implicated as etiological agents in sudden death syndrome in young domestic livestock. The main virulence factor produced by these strains is a zinc-dependent, phosphatidylcholine-preferring phospholipase C (alpha-toxin). The crystal structure of alpha-toxin, at pH 7.5, with the active site open and therefore accessible to substrate has previously been reported, as has calcium-binding to the C-terminal domain of the enzyme at pH 4.7. Here we focus on conformation changes in the N-terminal domain of alpha-toxin in crystals grown at acidic pH. These changes result in both the obscuring of the toxin active site and the loss of one of three zinc ions from it. Additionally, this "closed" form contains a small alpha helix, not present in the open structure, which hydrogen bonds to both the N and C-terminal domains. In conjunction with the previously reported findings that alpha-toxin can exist in active and inactive forms and that Thr74Ile and Phe69Cys substitutions markedly reduced the haemolytic activity of the enzyme, our work suggests that these loop conformations play a critical role in the activity of the toxin.
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(as it appears on PubMed at http://www.pubmed.gov), where 12051905 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12051905}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Zinc phospholipase c]]
[[Category: Zinc phospholipase c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:10:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:19:01 2008''

Revision as of 03:19, 1 July 2008

Image:1gyg.png

Template:STRUCTURE 1gyg

R32 CLOSED FORM OF ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS STRAIN CER89L43

Template:ABSTRACT PUBMED 12051905

About this Structure

1GYG is a Single protein structure of sequence from Clostridium perfringens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the C. perfringens alpha-toxin with the active site closed by a flexible loop region., Eaton JT, Naylor CE, Howells AM, Moss DS, Titball RW, Basak AK, J Mol Biol. 2002 May 31;319(2):275-81. PMID:12051905

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