7r5s
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ==== | + | ==Structure of the human CCAN bound to alpha satellite DNA== |
| - | <StructureSection load='7r5s' size='340' side='right'caption='[[7r5s]]' scene=''> | + | <StructureSection load='7r5s' size='340' side='right'caption='[[7r5s]], [[Resolution|resolution]] 2.83Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7r5s]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R5S FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r5s OCA], [https://pdbe.org/7r5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r5s RCSB], [https://www.ebi.ac.uk/pdbsum/7r5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r5s ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.83Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r5s OCA], [https://pdbe.org/7r5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r5s RCSB], [https://www.ebi.ac.uk/pdbsum/7r5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r5s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CENPH_HUMAN CENPH_HUMAN] Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.<ref>PMID:14536089</ref> <ref>PMID:16875666</ref> <ref>PMID:18007590</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A(Nuc)) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A(Nuc) reconstituted onto alpha-satellite DNA. CCAN forms edge-on contacts with CENP-A(Nuc), and a linker DNA segment of the alpha-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. | ||
| + | |||
| + | Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.,Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D Science. 2022 May 20;376(6595):844-852. doi: 10.1126/science.abn3810. Epub 2022 , Apr 14. PMID:35420891<ref>PMID:35420891</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7r5s" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Centromere protein 3D structure|Centromere protein 3D structure]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Z | + | [[Category: Barford D]] |
| + | [[Category: Bellini D]] | ||
| + | [[Category: Dendooven T]] | ||
| + | [[Category: McLaughlin SH]] | ||
| + | [[Category: Muir KW]] | ||
| + | [[Category: Predin M]] | ||
| + | [[Category: Tischer T]] | ||
| + | [[Category: Yang J]] | ||
| + | [[Category: Yatskevich S]] | ||
| + | [[Category: Zhang Z]] | ||
Current revision
Structure of the human CCAN bound to alpha satellite DNA
| |||||||||||
Categories: Homo sapiens | Large Structures | Barford D | Bellini D | Dendooven T | McLaughlin SH | Muir KW | Predin M | Tischer T | Yang J | Yatskevich S | Zhang Z
