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7q3a
From Proteopedia
(Difference between revisions)
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==Crystal structure of MAB_4324 a tandem repeat GNAT from Mycobacterium abscessus== | ==Crystal structure of MAB_4324 a tandem repeat GNAT from Mycobacterium abscessus== | ||
| - | <StructureSection load='7q3a' size='340' side='right'caption='[[7q3a]]' scene=''> | + | <StructureSection load='7q3a' size='340' side='right'caption='[[7q3a]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7q3a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3A FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3a OCA], [https://pdbe.org/7q3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3a RCSB], [https://www.ebi.ac.uk/pdbsum/7q3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3a ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3a OCA], [https://pdbe.org/7q3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3a RCSB], [https://www.ebi.ac.uk/pdbsum/7q3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycobacterium abscessus is a pathogenic non-tuberculous mycobacterium that possesses an intrinsic drug-resistance profile. Several N-acetyltransferases mediate drug resistance and/or participate in M. abscessus virulence. Mining the M. abscessus genome has revealed genes encoding additional N-acetyltransferases whose functions remain uncharacterized, among them MAB_4324c. Here, we showed that the purified MAB_4324c protein is a N-acetyltransferase able to acetylate small polyamine substrates. The crystal structure of MAB_4324c was solved at high resolution in complex with its cofactor, revealing the presence of two GCN5-related N-acetyltransferase domains and a cryptic binding site for NADPH. Genetic studies demonstrate that MAB_4324c is not essential for in vitro growth of M. abscessus, however overexpression of the protein enhanced the uptake and survival of M. abscessus in THP-1 macrophages. | ||
| + | |||
| + | Biochemical, structural, and functional studies reveal that MAB_4324c from Mycobacterium abscessus is an active tandem repeat N-acetyltransferase.,M A B Alsarraf H, Lam Ung K, Johansen MD, Dimon J, Olieric V, Kremer L, Blaise M FEBS Lett. 2022 Apr 25. doi: 10.1002/1873-3468.14360. PMID:35470425<ref>PMID:35470425</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7q3a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Alsarraf | + | [[Category: Alsarraf, M A.B]] |
| - | [[Category: Blaise M]] | + | [[Category: Blaise, M]] |
| + | [[Category: Acetyl-coa]] | ||
| + | [[Category: N-acetyltransferase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 16:22, 6 July 2022
Crystal structure of MAB_4324 a tandem repeat GNAT from Mycobacterium abscessus
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