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7tfs
From Proteopedia
(Difference between revisions)
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==Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens== | ==Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens== | ||
| - | <StructureSection load='7tfs' size='340' side='right'caption='[[7tfs]]' scene=''> | + | <StructureSection load='7tfs' size='340' side='right'caption='[[7tfs]], [[Resolution|resolution]] 4.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7tfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfs OCA], [https://pdbe.org/7tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfs RCSB], [https://www.ebi.ac.uk/pdbsum/7tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfs ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfs OCA], [https://pdbe.org/7tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfs RCSB], [https://www.ebi.ac.uk/pdbsum/7tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q74FJ0_GEOSL Q74FJ0_GEOSL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 A resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells. | ||
| + | |||
| + | Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.,Wang F, Mustafa K, Suciu V, Joshi K, Chan CH, Choi S, Su Z, Si D, Hochbaum AI, Egelman EH, Bond DR Nat Microbiol. 2022 Aug;7(8):1291-1300. doi: 10.1038/s41564-022-01159-z. Epub, 2022 Jul 7. PMID:35798889<ref>PMID:35798889</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7tfs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Geobacter sulfurreducens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bond DR]] | [[Category: Bond DR]] | ||
Current revision
Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens
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