|
|
| Line 3: |
Line 3: |
| | <SX load='3jc1' size='340' side='right' viewer='molstar' caption='[[3jc1]], [[Resolution|resolution]] 4.00Å' scene=''> | | <SX load='3jc1' size='340' side='right' viewer='molstar' caption='[[3jc1]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3jc1]] is a 68 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JC1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3jc1]] is a 68 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JC1 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IST1, KIAA0174 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), C18orf2, CHMP1B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc1 OCA], [https://pdbe.org/3jc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jc1 RCSB], [https://www.ebi.ac.uk/pdbsum/3jc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc1 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc1 OCA], [https://pdbe.org/3jc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jc1 RCSB], [https://www.ebi.ac.uk/pdbsum/3jc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/IST1_HUMAN IST1_HUMAN]] Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.<ref>PMID:19129479</ref> <ref>PMID:19129480</ref> [[https://www.uniprot.org/uniprot/CHM1B_HUMAN CHM1B_HUMAN]] Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.<ref>PMID:14519844</ref> <ref>PMID:19129479</ref>
| + | [https://www.uniprot.org/uniprot/IST1_HUMAN IST1_HUMAN] Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.<ref>PMID:19129479</ref> <ref>PMID:19129480</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The Endosomal Sorting Complexes Required for Transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 A resolution cryo-EM reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, CHMP1B and IST1. The inner strand comprises "open" CHMP1B subunits that interlock in an elaborate domain-swapped architecture, and is encircled by an outer strand of "closed" IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively-curved membranes in vitro and in vivo. Our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.
| + | |
| - | | + | |
| - | Structure and membrane remodeling activity of ESCRT-III helical polymers.,McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A Science. 2015 Dec 3. pii: aad8305. PMID:26634441<ref>PMID:26634441</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3jc1" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| Line 26: |
Line 17: |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Afonine, P]] | + | [[Category: Afonine P]] |
| - | [[Category: Arthur, C]] | + | [[Category: Arthur C]] |
| - | [[Category: Clippinger, A K]] | + | [[Category: Clippinger AK]] |
| - | [[Category: Colf, L A]] | + | [[Category: Colf LA]] |
| - | [[Category: Frost, A]] | + | [[Category: Frost A]] |
| - | [[Category: Hanson, P I]] | + | [[Category: Hanson PI]] |
| - | [[Category: McCullough, J]] | + | [[Category: McCullough J]] |
| - | [[Category: Naismith, T V]] | + | [[Category: Naismith TV]] |
| - | [[Category: Saunders, M G]] | + | [[Category: Saunders MG]] |
| - | [[Category: Skowyra, M L]] | + | [[Category: Skowyra ML]] |
| - | [[Category: Sundquist, W I]] | + | [[Category: Sundquist WI]] |
| - | [[Category: Talledge, N]] | + | [[Category: Talledge N]] |
| - | [[Category: Chmp1b]]
| + | |
| - | [[Category: Escrt-iii]]
| + | |
| - | [[Category: Helical filament]]
| + | |
| - | [[Category: Ist1]]
| + | |
| - | [[Category: Lipid binding protein]]
| + | |
| - | [[Category: Membrane tubulation]]
| + | |
