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| | <StructureSection load='3l1p' size='340' side='right'caption='[[3l1p]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='3l1p' size='340' side='right'caption='[[3l1p]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3l1p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3l1p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L1P FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PO5F1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l1p OCA], [https://pdbe.org/3l1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l1p RCSB], [https://www.ebi.ac.uk/pdbsum/3l1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l1p ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l1p OCA], [https://pdbe.org/3l1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l1p RCSB], [https://www.ebi.ac.uk/pdbsum/3l1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l1p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PO5F1_MOUSE PO5F1_MOUSE]] Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.<ref>PMID:17496161</ref> <ref>PMID:17525163</ref>
| + | [https://www.uniprot.org/uniprot/PO5F1_MOUSE PO5F1_MOUSE] Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.<ref>PMID:17496161</ref> <ref>PMID:17525163</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Groves, M R]] | + | [[Category: Groves MR]] |
| - | [[Category: Pogenberg, V]] | + | [[Category: Pogenberg V]] |
| - | [[Category: Vahokoski, J]] | + | [[Category: Vahokoski J]] |
| - | [[Category: Wilmanns, M]] | + | [[Category: Wilmanns M]] |
| - | [[Category: Embryonic]]
| + | |
| - | [[Category: Pore]]
| + | |
| - | [[Category: Pou]]
| + | |
| - | [[Category: Stem cell]]
| + | |
| - | [[Category: Transcription factor dna complex]]
| + | |
| - | [[Category: Transcription-dna complex]]
| + | |
| Structural highlights
Function
PO5F1_MOUSE Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.[1] [2]
Publication Abstract from PubMed
Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an alpha-helix and exposed to the protein's surface, in contrast to the unstructured linker of Oct1. Point mutations in this alpha-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein-protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process.
A unique Oct4 interface is crucial for reprogramming to pluripotency.,Esch D, Vahokoski J, Groves MR, Pogenberg V, Cojocaru V, Vom Bruch H, Han D, Drexler HC, Arauzo-Bravo MJ, Ng CK, Jauch R, Wilmanns M, Scholer HR Nat Cell Biol. 2013 Mar;15(3):295-301. doi: 10.1038/ncb2680. Epub 2013 Feb 3. PMID:23376973[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhang Z, Liao B, Xu M, Jin Y. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1. FASEB J. 2007 Oct;21(12):3042-51. Epub 2007 May 11. PMID:17496161 doi:fj.06-6914com
- ↑ Wei F, Scholer HR, Atchison ML. Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation. J Biol Chem. 2007 Jul 20;282(29):21551-60. Epub 2007 May 24. PMID:17525163 doi:M611041200
- ↑ Esch D, Vahokoski J, Groves MR, Pogenberg V, Cojocaru V, Vom Bruch H, Han D, Drexler HC, Arauzo-Bravo MJ, Ng CK, Jauch R, Wilmanns M, Scholer HR. A unique Oct4 interface is crucial for reprogramming to pluripotency. Nat Cell Biol. 2013 Mar;15(3):295-301. doi: 10.1038/ncb2680. Epub 2013 Feb 3. PMID:23376973 doi:10.1038/ncb2680
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