1h5c
From Proteopedia
(New page: 200px<br /> <applet load="1h5c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h5c, resolution 1.62Å" /> '''X-RAY INDUCED REDUC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H5C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]] with ACT, CA and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5C OCA]]. | + | 1H5C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]] with ACT, CA and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Peroxidase Peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]]. Structure known Active Site: CAA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5C OCA]]. |
==Reference== | ==Reference== | ||
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12024218 12024218] | The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12024218 12024218] | ||
[[Category: Armoracia rusticana]] | [[Category: Armoracia rusticana]] | ||
| + | [[Category: Peroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Berglund, G.I.]] | [[Category: Berglund, G.I.]] | ||
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[[Category: x-ray induced reduction]] | [[Category: x-ray induced reduction]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:34:57 2007'' |
Revision as of 09:30, 30 October 2007
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X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (100-200% DOSE)
Overview
A molecular description of oxygen and peroxide activation in biological, systems is difficult, because electrons liberated during X-ray data, collection reduce the active centres of redox enzymes catalysing these, reactions. Here we describe an effective strategy to obtain crystal, structures for high-valency redox intermediates and present a, three-dimensional movie of the X-ray-driven catalytic reduction of a bound, dioxygen species in horseradish peroxidase (HRP). We also describe, separate experiments in which high-resolution structures could be obtained, for all five oxidation states of HRP, showing such structures with, preserved redox states for the first time.
About this Structure
1H5C is a [Single protein] structure of sequence from [Armoracia rusticana] with ACT, CA and HEM as [ligands]. Active as [Peroxidase], with EC number [1.11.1.7]. Structure known Active Site: CAA. Full crystallographic information is available from [OCA].
Reference
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:12024218
Page seeded by OCA on Tue Oct 30 11:34:57 2007
