1h38

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[[Image:1h38.gif|left|200px]]
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{{Seed}}
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[[Image:1h38.png|left|200px]]
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{{STRUCTURE_1h38| PDB=1h38 | SCENE= }}
{{STRUCTURE_1h38| PDB=1h38 | SCENE= }}
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'''STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION'''
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===STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION===
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==Overview==
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The single-subunit bacteriophage T7 RNA polymerase carries out the transcription cycle in an identical manner to that of bacterial and eukaryotic multisubunit enzymes. Here we report the crystal structure of a T7 RNA polymerase elongation complex, which shows that incorporation of an 8-base-pair RNA-DNA hybrid into the active site of the enzyme induces a marked rearrangement of the amino-terminal domain. This rearrangement involves alternative folding of about 130 residues and a marked reorientation (about 130 degrees rotation) of a stable core subdomain, resulting in a structure that provides elements required for stable transcription elongation. A wide opening on the enzyme surface that is probably an RNA exit pathway is formed, and the RNA-DNA hybrid is completely buried in a newly formed, deep protein cavity. Binding of 10 base pairs of downstream DNA is stabilized mostly by long-distance electrostatic interactions. The structure implies plausible mechanisms for the various phases of the transcription cycle, and reveals important structural similarities with the multisubunit RNA polymerases.
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The line below this paragraph, {{ABSTRACT_PUBMED_12422209}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12422209 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12422209}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: T7 rna polymerase]]
[[Category: T7 rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:22:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:33:04 2008''

Revision as of 03:33, 1 July 2008

Image:1h38.png

Template:STRUCTURE 1h38

STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION

Template:ABSTRACT PUBMED 12422209

About this Structure

1H38 is a Protein complex structure of sequences from Enterobacteria phage t7. Full crystallographic information is available from OCA.

Reference

Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution., Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S, Nature. 2002 Nov 7;420(6911):43-50. Epub 2002 Oct 9. PMID:12422209

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