3mwp

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Current revision (09:58, 14 February 2024) (edit) (undo)
 
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<StructureSection load='3mwp' size='340' side='right'caption='[[3mwp]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
<StructureSection load='3mwp' size='340' side='right'caption='[[3mwp]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3mwp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassj Lassj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MWP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3mwp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_virus_Josiah Lassa virus Josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MWP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.795&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3mwt|3mwt]], [[3mx2|3mx2]], [[3mx5|3mx5]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N, Nucleoprotein ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11622 LASSJ])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mwp OCA], [https://pdbe.org/3mwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mwp RCSB], [https://www.ebi.ac.uk/pdbsum/3mwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mwp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mwp OCA], [https://pdbe.org/3mwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mwp RCSB], [https://www.ebi.ac.uk/pdbsum/3mwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mwp ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).
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[https://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lassa virus, the causative agent of Lassa fever, causes thousands of deaths annually and is a biological threat agent, for which there is no vaccine and limited therapy. The nucleoprotein (NP) of Lassa virus has essential roles in viral RNA synthesis and immune suppression, the molecular mechanisms of which are poorly understood. Here we report the crystal structure of Lassa virus NP at 1.80 A resolution, which reveals amino (N)- and carboxy (C)-terminal domains with structures unlike any of the reported viral NPs. The N domain folds into a novel structure with a deep cavity for binding the m7GpppN cap structure that is required for viral RNA transcription, whereas the C domain contains 3'-5' exoribonuclease activity involved in suppressing interferon induction. To our knowledge this is the first X-ray crystal structure solved for an arenaviral NP, which reveals its unexpected functions and indicates unique mechanisms in cap binding and immune evasion. These findings provide great potential for vaccine and drug development.
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Cap binding and immune evasion revealed by Lassa nucleoprotein structure.,Qi X, Lan S, Wang W, Schelde LM, Dong H, Wallat GD, Ly H, Liang Y, Dong C Nature. 2010 Nov 17. PMID:21085117<ref>PMID:21085117</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3mwp" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lassj]]
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[[Category: Lassa virus Josiah]]
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[[Category: Dong, C]]
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[[Category: Dong C]]
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[[Category: Dong, H]]
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[[Category: Dong H]]
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[[Category: Lan, S]]
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[[Category: Lan S]]
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[[Category: Liang, Y]]
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[[Category: Liang Y]]
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[[Category: Ly, H]]
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[[Category: Ly H]]
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[[Category: Qi, X]]
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[[Category: Qi X]]
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[[Category: SSPF, Scottish Structural Proteomics Facility]]
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[[Category: Schelde LM]]
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[[Category: Schelde, L M]]
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[[Category: Wallat G]]
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[[Category: Wallat, G]]
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[[Category: Wang W]]
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[[Category: Wang, W]]
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[[Category: Nuclear protein]]
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[[Category: Nucleoprotein]]
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[[Category: Scottish structural proteomics facility]]
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[[Category: Sspf]]
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[[Category: Structural genomic]]
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Current revision

Nucleoprotein structure of lassa fever virus

PDB ID 3mwp

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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