7zu0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==HOPS tethering complex from yeast== | |
| + | <StructureSection load='7zu0' size='340' side='right'caption='[[7zu0]], [[Resolution|resolution]] 4.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7zu0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZU0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zu0 OCA], [https://pdbe.org/7zu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zu0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zu0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/PEP5_YEAST PEP5_YEAST]] Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion (PubMed:3062374, PubMed:10978279, PubMed:10944212, PubMed:16601699). Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21 (PubMed:17488625). Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation (PubMed:22570702).<ref>PMID:10944212</ref> <ref>PMID:10978279</ref> <ref>PMID:16601699</ref> <ref>PMID:17488625</ref> <ref>PMID:22570702</ref> <ref>PMID:3062374</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lysosomes are essential for cellular recycling, nutrient signaling, autophagy, and pathogenic bacteria and viruses invasion. Lysosomal fusion is fundamental to cell survival and requires HOPS, a conserved heterohexameric tethering complex. On the membranes to be fused, HOPS binds small membrane-associated GTPases and assembles SNAREs for fusion, but how the complex fulfills its function remained speculative. Here, we used cryo-electron microscopy to reveal the structure of HOPS. Unlike previously reported, significant flexibility of HOPS is confined to its extremities, where GTPase binding occurs. The SNARE-binding module is firmly attached to the core, therefore, ideally positioned between the membranes to catalyze fusion. Our data suggest a model for how HOPS fulfills its dual functionality of tethering and fusion and indicate why it is an essential part of the membrane fusion machinery. | ||
| - | + | Structure of the HOPS tethering complex, a lysosomal membrane fusion machinery.,Shvarev D, Schoppe J, Konig C, Perz A, Fullbrunn N, Kiontke S, Langemeyer L, Januliene D, Schnelle K, Kummel D, Frohlich F, Moeller A, Ungermann C Elife. 2022 Sep 13;11. pii: 80901. doi: 10.7554/eLife.80901. PMID:36098503<ref>PMID:36098503</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7zu0" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Kiontke | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: | + | [[Category: Froehlich F]] |
| - | [[Category: Moeller | + | [[Category: Fuellbrunn N]] |
| - | [[Category: Perz | + | [[Category: Januliene D]] |
| - | [[Category: | + | [[Category: Kiontke S]] |
| - | [[Category: Schoppe | + | [[Category: Koenig C]] |
| - | [[Category: Ungermann | + | [[Category: Kuemmel D]] |
| + | [[Category: Langemeyer L]] | ||
| + | [[Category: Moeller A]] | ||
| + | [[Category: Perz A]] | ||
| + | [[Category: Schnelle K]] | ||
| + | [[Category: Schoppe J]] | ||
| + | [[Category: Shvarev D]] | ||
| + | [[Category: Ungermann C]] | ||
Revision as of 06:21, 28 September 2022
HOPS tethering complex from yeast
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Categories: Large Structures | Saccharomyces cerevisiae | Froehlich F | Fuellbrunn N | Januliene D | Kiontke S | Koenig C | Kuemmel D | Langemeyer L | Moeller A | Perz A | Schnelle K | Schoppe J | Shvarev D | Ungermann C
