7rcj
From Proteopedia
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==Crystal structure of ZnuA from Citrobacter koseri== | ==Crystal structure of ZnuA from Citrobacter koseri== | ||
| - | <StructureSection load='7rcj' size='340' side='right'caption='[[7rcj]]' scene=''> | + | <StructureSection load='7rcj' size='340' side='right'caption='[[7rcj]], [[Resolution|resolution]] 3.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RCJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7rcj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_koseri_ATCC_BAA-895 Citrobacter koseri ATCC BAA-895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RCJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rcj OCA], [https://pdbe.org/7rcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rcj RCSB], [https://www.ebi.ac.uk/pdbsum/7rcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rcj ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rcj OCA], [https://pdbe.org/7rcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rcj RCSB], [https://www.ebi.ac.uk/pdbsum/7rcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rcj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A8AFI6_CITK8 A8AFI6_CITK8] Involved in the high-affinity zinc uptake transport system.[ARBA:ARBA00002313] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Zinc is an essential metal for all kingdoms of life, making its transport across the cell membrane a critical function. In bacteria, high-affinity zinc import is accomplished by ATP-binding cassette (ABC) transporters, which rely on extracellular solute-binding proteins (SBPs) of cluster A-I to acquire the metal and deliver it to the membrane permease. These systems are important for survival and virulence, making them attractive targets for the development of novel antibiotics. Citrobacter koseri is an emerging pathogen with extensive antibiotic resistance. High-affinity zinc binding to the C. koseri cluster A-I SBP ZnuA has been characterized and the structure of the zinc-bound (holo) form has been determined by X-ray crystallography. Remarkably, despite 95% sequence identity to the ZnuA homologue from Salmonella enterica, C. koseri ZnuA exhibits a different zinc-coordination environment and a closed rather than an open conformation. Comparison with structures of another close ZnuA homologue from Escherichia coli suggests a surprisingly flexible conformational landscape that may be important for efficient zinc binding and/or delivery to the membrane permease. | ||
| + | |||
| + | Conformational flexibility in the zinc solute-binding protein ZnuA.,Yekwa EL, Serrano FA, Yukl E Acta Crystallogr F Struct Biol Commun. 2022 Mar 1;78(Pt 3):128-134. doi:, 10.1107/S2053230X22001662. Epub 2022 Feb 28. PMID:35234138<ref>PMID:35234138</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7rcj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Citrobacter koseri ATCC BAA-895]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Yekwa EL]] | [[Category: Yekwa EL]] | ||
[[Category: Yukl ET]] | [[Category: Yukl ET]] | ||
Revision as of 16:28, 18 October 2023
Crystal structure of ZnuA from Citrobacter koseri
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