7ek1

<table><tr><td colspan='2'>[[7ek1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EK1 FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ek1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ek1 OCA], [https://pdbe.org/7ek1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ek1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ek1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ek1 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

In the light reaction of plant photosynthesis, modulation of electron transport chain reactions is important to maintain the efficiency of photosynthesis under a broad range of light intensities. VCCN1 was recently identified as a voltage-gated chloride channel residing in the thylakoid membrane, where it plays a key role in photoreaction tuning to avoid the generation of reactive oxygen species (ROS). Here, we present the cryo-EM structures of Malus domestica VCCN1 (MdVCCN1) in nanodiscs and detergent at 2.7 A and 3.0 A resolutions, respectively, and the structure-based electrophysiological analyses. VCCN1 structurally resembles its animal homolog, bestrophin, a Ca(2+)-gated anion channel. However, unlike bestrophin channels, VCCN1 lacks the Ca(2+)-binding motif but instead contains an N-terminal charged helix that is anchored to the lipid membrane through an additional amphipathic helix. Electrophysiological experiments demonstrate that these structural elements are essential for the channel activity, thus revealing the distinct activation mechanism of VCCN1.

 

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== References ==

[[Category: Hagino, T]]

[[Category: Kasuya, G]]

[[Category: Kato, T]]

[[Category: Kobayashi, K]]

[[Category: Kusakizako, T]]

[[Category: Nishizawa, T]]

[[Category: Nureki, O]]

[[Category: Yamashita, K]]

[[Category: Thylakoid]]