3omu

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of the N-terminal domain of an HSP90 from Trypanosoma Brucei, Tb10.26.1080 in the presence of a thienopyrimidine derivative

Structural highlights

3omu is a 2 chain structure with sequence from Trypanosoma brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.15Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q389P1_TRYB2

Publication Abstract from PubMed

Human African trypanosomiasis is a neglected parasitic disease that is fatal if untreated. The current drugs available to eliminate the causative agent Trypanosoma brucei have multiple liabilities, including toxicity, increasing problems due to treatment failure and limited efficacy. There are two approaches to discover novel antimicrobial drugs--whole-cell screening and target-based discovery. In the latter case, there is a need to identify and validate novel drug targets in Trypanosoma parasites. The heat shock proteins (Hsp), while best known as cancer targets with a number of drug candidates in clinical development, are a family of emerging targets for infectious diseases. In this paper, we report the exploration of T. brucei Hsp83--a homolog of human Hsp90--as a drug target using multiple biophysical and biochemical techniques. Our approach included the characterization of the chemical sensitivity of the parasitic chaperone against a library of known Hsp90 inhibitors by means of differential scanning fluorimetry (DSF). Several compounds identified by this screening procedure were further studied using isothermal titration calorimetry (ITC) and X-ray crystallography, as well as tested in parasite growth inhibitions assays. These experiments led us to the identification of a benzamide derivative compound capable of interacting with TbHsp83 more strongly than with its human homologs and structural rationalization of this selectivity. The results highlight the opportunities created by subtle structural differences to develop new series of compounds to selectively target the Trypanosoma brucei chaperone and effectively kill the sleeping sickness parasite.

Exploring the Trypanosoma brucei Hsp83 potential as a target for structure guided drug design.,Pizarro JC, Hills T, Senisterra G, Wernimont AK, Mackenzie C, Norcross NR, Ferguson MA, Wyatt PG, Gilbert IH, Hui R PLoS Negl Trop Dis. 2013 Oct 17;7(10):e2492. doi: 10.1371/journal.pntd.0002492., eCollection 2013. PMID:24147171^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pizarro JC, Hills T, Senisterra G, Wernimont AK, Mackenzie C, Norcross NR, Ferguson MA, Wyatt PG, Gilbert IH, Hui R. Exploring the Trypanosoma brucei Hsp83 potential as a target for structure guided drug design. PLoS Negl Trop Dis. 2013 Oct 17;7(10):e2492. doi: 10.1371/journal.pntd.0002492., eCollection 2013. PMID:24147171 doi:http://dx.doi.org/10.1371/journal.pntd.0002492

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3omu"

@@ Line 3: / Line 3: @@
 <StructureSection load='3omu' size='340' side='right'caption='[[3omu]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3omu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OMU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3omu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OMU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IBD:2-AMINO-4-{2,4-DICHLORO-5-[2-(DIETHYLAMINO)ETHOXY]PHENYL}-N-ETHYLTHIENO[2,3-D]PYRIMIDINE-6-CARBOXAMIDE'>IBD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o6o|3o6o]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IBD:2-AMINO-4-{2,4-DICHLORO-5-[2-(DIETHYLAMINO)ETHOXY]PHENYL}-N-ETHYLTHIENO[2,3-D]PYRIMIDINE-6-CARBOXAMIDE'>IBD</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tb10.26.1080 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma (Trypanozoon) brucei])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3omu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3omu OCA], [https://pdbe.org/3omu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3omu RCSB], [https://www.ebi.ac.uk/pdbsum/3omu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3omu ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q389P1_TRYB2 Q389P1_TRYB2]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Arrowsmith, C H]]
+[[Category: Trypanosoma brucei]]
-[[Category: Bochkarev, A]]
+[[Category: Arrowsmith CH]]
-[[Category: Bountra, C]]
+[[Category: Bochkarev A]]
-[[Category: Cossar, D]]
+[[Category: Bountra C]]
-[[Category: Edwards, A M]]
+[[Category: Cossar D]]
-[[Category: Fairlamb, A H]]
+[[Category: Edwards AM]]
-[[Category: Ferguson, M A.J]]
+[[Category: Fairlamb AH]]
-[[Category: Hills, T]]
+[[Category: Ferguson MAJ]]
-[[Category: Hui, R]]
+[[Category: Hills T]]
-[[Category: Hutchinson, A]]
+[[Category: Hui R]]
-[[Category: Kozieradzki, I]]
+[[Category: Hutchinson A]]
-[[Category: Li, Y]]
+[[Category: Kozieradzki I]]
-[[Category: MacKenzie, C]]
+[[Category: Li Y]]
-[[Category: Pizarro, J C]]
+[[Category: MacKenzie C]]
-[[Category: Structural genomic]]
+[[Category: Pizarro JC]]
-[[Category: Sullivan, H]]
+[[Category: Sullivan H]]
-[[Category: Weadge, J]]
+[[Category: Weadge J]]
-[[Category: Weigelt, J]]
+[[Category: Weigelt J]]
-[[Category: Wernimont, A K]]
+[[Category: Wernimont AK]]
-[[Category: Wyatt, P G]]
+[[Category: Wyatt PG]]
-[[Category: Chaperone]]
-[[Category: Drug discovery]]
-[[Category: Hsp90]]
-[[Category: Sgc]]
-[[Category: Trypanosoma bruceii]]

3omu

From Proteopedia

Current revision

Crystal Structure of the N-terminal domain of an HSP90 from Trypanosoma Brucei, Tb10.26.1080 in the presence of a thienopyrimidine derivative

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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