1h6s

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[[Image:1h6s.gif|left|200px]]
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{{Seed}}
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[[Image:1h6s.png|left|200px]]
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{{STRUCTURE_1h6s| PDB=1h6s | SCENE= }}
{{STRUCTURE_1h6s| PDB=1h6s | SCENE= }}
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'''ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS'''
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===ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS===
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==Overview==
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Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.
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The line below this paragraph, {{ABSTRACT_PUBMED_12468713}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12468713 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12468713}}
==About this Structure==
==About this Structure==
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[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
[[Category: Integral membrane protein porin]]
[[Category: Integral membrane protein porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:30:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:43:05 2008''

Revision as of 03:43, 1 July 2008

Image:1h6s.png

Template:STRUCTURE 1h6s

ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS

Template:ABSTRACT PUBMED 12468713

About this Structure

1H6S is a Single protein structure of sequence from Rhodobacter blasticus. Full crystallographic information is available from OCA.

Reference

Asymmetric conductivity of engineered porins., Bannwarth M, Schulz GE, Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713

Page seeded by OCA on Tue Jul 1 06:43:05 2008

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