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| | <StructureSection load='3dix' size='340' side='right'caption='[[3dix]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='3dix' size='340' side='right'caption='[[3dix]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3dix]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3dix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3dig|3dig]], [[3dil|3dil]], [[3dim|3dim]], [[3dio|3dio]], [[3diq|3diq]], [[3dir|3dir]], [[3dis|3dis]], [[3diy|3diy]], [[3diz|3diz]], [[3dj0|3dj0]], [[3dj2|3dj2]]</div></td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dix OCA], [https://pdbe.org/3dix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dix RCSB], [https://www.ebi.ac.uk/pdbsum/3dix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dix ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dix OCA], [https://pdbe.org/3dix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dix RCSB], [https://www.ebi.ac.uk/pdbsum/3dix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dix ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Serganov, A A]] | + | [[Category: Thermotoga maritima]] |
| - | [[Category: Lysine]] | + | [[Category: Serganov AA]] |
| - | [[Category: Potassium cation]]
| + | |
| - | [[Category: Riboswitch]]
| + | |
| - | [[Category: Rna]]
| + | |
| Structural highlights
Publication Abstract from PubMed
In bacteria, the intracellular concentration of several amino acids is controlled by riboswitches. One of the important regulatory circuits involves lysine-specific riboswitches, which direct the biosynthesis and transport of lysine and precursors common for lysine and other amino acids. To understand the molecular basis of amino acid recognition by riboswitches, here we present the crystal structure of the 174-nucleotide sensing domain of the Thermotoga maritima lysine riboswitch in the lysine-bound (1.9 angstrom (A)) and free (3.1 A) states. The riboswitch features an unusual and intricate architecture, involving three-helical and two-helical bundles connected by a compact five-helical junction and stabilized by various long-range tertiary interactions. Lysine interacts with the junctional core of the riboswitch and is specifically recognized through shape-complementarity within the elongated binding pocket and through several direct and K(+)-mediated hydrogen bonds to its charged ends. Our structural and biochemical studies indicate preformation of the riboswitch scaffold and identify conformational changes associated with the formation of a stable lysine-bound state, which prevents alternative folding of the riboswitch and facilitates formation of downstream regulatory elements. We have also determined several structures of the riboswitch bound to different lysine analogues, including antibiotics, in an effort to understand the ligand-binding capabilities of the lysine riboswitch and understand the nature of antibiotic resistance. Our results provide insights into a mechanism of lysine-riboswitch-dependent gene control at the molecular level, thereby contributing to continuing efforts at exploration of the pharmaceutical and biotechnological potential of riboswitches.
Structural insights into amino acid binding and gene control by a lysine riboswitch.,Serganov A, Huang L, Patel DJ Nature. 2008 Oct 30;455(7217):1263-7. Epub 2008 Sep 10. PMID:18784651[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Serganov A, Huang L, Patel DJ. Structural insights into amino acid binding and gene control by a lysine riboswitch. Nature. 2008 Oct 30;455(7217):1263-7. Epub 2008 Sep 10. PMID:18784651 doi:http://dx.doi.org/nature07326
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