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3dw7
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3dw7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3dw7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW7 FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw7 OCA], [https://pdbe.org/3dw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw7 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw7 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw7 OCA], [https://pdbe.org/3dw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw7 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality. | ||
| - | |||
| - | A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3dw7" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Dumas | + | [[Category: Dumas P]] |
| - | [[Category: Ennifar | + | [[Category: Ennifar E]] |
| - | [[Category: Lang | + | [[Category: Lang K]] |
| - | [[Category: Micura | + | [[Category: Micura R]] |
| - | [[Category: Olieric | + | [[Category: Olieric V]] |
| - | [[Category: Rieder | + | [[Category: Rieder U]] |
| - | [[Category: Schulze-Briese | + | [[Category: Schulze-Briese C]] |
| - | [[Category: Serganov | + | [[Category: Serganov A]] |
| - | + | ||
| - | + | ||
Current revision
Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2656-SeCH3 modified
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Categories: Large Structures | Dumas P | Ennifar E | Lang K | Micura R | Olieric V | Rieder U | Schulze-Briese C | Serganov A
