3pp5

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Current revision (10:42, 21 February 2024) (edit) (undo)
 
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<StructureSection load='3pp5' size='340' side='right'caption='[[3pp5]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='3pp5' size='340' side='right'caption='[[3pp5]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [https://pdbe.org/3pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [https://pdbe.org/3pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI]] Involved in regulation of actin and microtubule organization.
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[https://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI] Involved in regulation of actin and microtubule organization.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Scar/WAVE-complex links upstream Rho-GTPase signaling to the activation of the conserved Arp2/3-complex. Scar/WAVE-induced and Arp2/3-complex-mediated actin nucleation is crucial for actin assembly in protruding lamellipodia to drive cell migration. The heteropentameric Scar/WAVE-complex is composed of Scar/WAVE, Abi, Nap, Pir and a small polypeptide Brk1/HSPC300, and recent work suggested that free Brk1 serves as a homooligomeric precursor in the assembly of this complex. Here we characterized the Brk1 trimer from Dictyostelium by analytical ultracentrifugation and gelfiltration. We show for the first time its dissociation at concentrations in the nanomolar range as well as an exchange of subunits within different DdBrk1 containing complexes. Moreover, we determined the three-dimensional structure of DdBrk1 at 1.5 A resolution by X-ray crystallography. Three chains of DdBrk1 are associated with each other forming a parallel triple coiled-coil bundle. Notably, this structure is highly similar to the heterotrimeric alpha-helical bundle of HSPC300/WAVE1/Abi2 within the human Scar/WAVE-complex. This finding, together with the fact that Brk1 is collectively sandwiched by the remaining subunits and also constitutes the main subunit connecting the triple-coil domain of the HSPC300/WAVE1/Abi2/ heterotrimer to Sra1(Pir1), implies a critical function of this subunit in the assembly process of the entire Scar/WAVE-complex.
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High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.,Linkner J, Witte G, Stradal T, Curth U, Faix J PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20. PMID:21701600<ref>PMID:21701600</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pp5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 11735]]
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[[Category: Dictyostelium discoideum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Curth, U]]
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[[Category: Curth U]]
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[[Category: Faix, J]]
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[[Category: Faix J]]
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[[Category: Linkner, J]]
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[[Category: Linkner J]]
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[[Category: Witte, G]]
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[[Category: Witte G]]
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[[Category: Abi]]
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[[Category: Precursor of the scar-wave complex]]
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[[Category: Scar]]
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[[Category: Structural protein]]
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[[Category: Triple coiled-coil]]
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Current revision

High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1

PDB ID 3pp5

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OCA

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