3pwf

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Current revision (10:43, 21 February 2024) (edit) (undo)
 
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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3pwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PWF FirstGlance]. <br>
<table><tr><td colspan='2'>[[3pwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PWF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwf OCA], [https://pdbe.org/3pwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pwf RCSB], [https://www.ebi.ac.uk/pdbsum/3pwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwf OCA], [https://pdbe.org/3pwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pwf RCSB], [https://www.ebi.ac.uk/pdbsum/3pwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwf ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9UWP7_9EURY Q9UWP7_9EURY]
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High-resolution crystal structures of Pyrococcus furiosus rubrerythrin (PfRbr) in the resting (all-ferrous) state and at time points following exposure of the crystals to hydrogen peroxide are reported. This approach was possible because of the relativity slow turnover of PfRbr at room temperature. To this end, we were able to perform time-dependent peroxide treatment of the fully reduced enzyme, under strictly anaerobic conditions, in the crystalline state. In this work we demonstrate, for the first time, that turnover of a thermophilic rubrerythrin results in approximately 2-A movement of one iron atom in the diiron site from a histidine to a carboxylate ligand. These results confirm that, despite the domain-swapped architecture, the hyperthermophilic rubrerythrins also utilize the classic combination of iron sites together with redox-dependent iron toggling to selectively reduce hydrogen peroxide over dioxygen. In addition, we have identified previously unobserved intermediates in the reaction cycle and observed structural changes that may explain the enzyme precipitation observed for the all-iron form of PfRbr upon oxidation to the all-ferric state.
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A cryo-crystallographic time course for peroxide reduction by rubrerythrin from Pyrococcus furiosus.,Dillard BD, Demick JM, Adams MW, Lanzilotta WN J Biol Inorg Chem. 2011 Jun 7. PMID:21647777<ref>PMID:21647777</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pwf" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Adams, M W]]
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[[Category: Adams MW]]
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[[Category: Demick, J M]]
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[[Category: Demick JM]]
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[[Category: Dillard, B D]]
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[[Category: Dillard BD]]
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[[Category: Lanzilotta, W N]]
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[[Category: Lanzilotta WN]]
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[[Category: Non heme iron peroxidase]]
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[[Category: Oxidative stress]]
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[[Category: Oxidoreductase]]
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[[Category: Rubrerythrin]]
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Current revision

High resolution structure of the fully reduced form of rubrerythrin from P. furiosus

PDB ID 3pwf

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