1f5q
From Proteopedia
(New page: 200px<br /> <applet load="1f5q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5q, resolution 2.50Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1f5q.gif|left|200px]]<br /> | + | [[Image:1f5q.gif|left|200px]]<br /><applet load="1f5q" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1f5q" size=" | + | |
caption="1f5q, resolution 2.50Å" /> | caption="1f5q, resolution 2.50Å" /> | ||
'''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''<br /> | '''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Several gamma-herpesviruses encode proteins related to the mammalian | + | Several gamma-herpesviruses encode proteins related to the mammalian cyclins, regulatory subunits of cyclin-dependent kinases (cdks) essential for cell cycle progression. We report a 2.5 A crystal structure of a full-length oncogenic viral cyclin from gamma-herpesvirus 68 complexed with cdk2. The viral cyclin binds cdk2 with an orientation different from cyclin A and makes several novel interactions at the interface, yet it activates cdk2 by triggering conformational changes similar to cyclin A. Sequences within the viral cyclin N-terminus lock part of the cdk2 T-loop within the core of the complex. These sequences and others are conserved amongst the viral and cellular D-type cyclins, suggesting that this structure has wider implications for other cyclin-cdk complexes. The observed resistance of this viral cyclin-cdk complex to inhibition by the p27(KIP:) cdk inhibitor is explained by sequence and conformational variation in the cyclin rendering the p27(KIP:)-binding site on the cyclin subunit non-functional. |
==About this Structure== | ==About this Structure== | ||
| - | 1F5Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1F5Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Murid herpesvirus 1]] | [[Category: Murid herpesvirus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Card, G | + | [[Category: Card, G L.]] |
[[Category: Jones, N.]] | [[Category: Jones, N.]] | ||
[[Category: Knowles, P.]] | [[Category: Knowles, P.]] | ||
[[Category: Laman, H.]] | [[Category: Laman, H.]] | ||
| - | [[Category: McDonald, N | + | [[Category: McDonald, N Q.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: cyclin dependent kinase. protein/protein complex]] | [[Category: cyclin dependent kinase. protein/protein complex]] | ||
[[Category: herpesviral cyclin]] | [[Category: herpesviral cyclin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:30 2008'' |
Revision as of 10:35, 21 February 2008
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CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2
Overview
Several gamma-herpesviruses encode proteins related to the mammalian cyclins, regulatory subunits of cyclin-dependent kinases (cdks) essential for cell cycle progression. We report a 2.5 A crystal structure of a full-length oncogenic viral cyclin from gamma-herpesvirus 68 complexed with cdk2. The viral cyclin binds cdk2 with an orientation different from cyclin A and makes several novel interactions at the interface, yet it activates cdk2 by triggering conformational changes similar to cyclin A. Sequences within the viral cyclin N-terminus lock part of the cdk2 T-loop within the core of the complex. These sequences and others are conserved amongst the viral and cellular D-type cyclins, suggesting that this structure has wider implications for other cyclin-cdk complexes. The observed resistance of this viral cyclin-cdk complex to inhibition by the p27(KIP:) cdk inhibitor is explained by sequence and conformational variation in the cyclin rendering the p27(KIP:)-binding site on the cyclin subunit non-functional.
About this Structure
1F5Q is a Protein complex structure of sequences from Homo sapiens and Murid herpesvirus 1 with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a gamma-herpesvirus cyclin-cdk complex., Card GL, Knowles P, Laman H, Jones N, McDonald NQ, EMBO J. 2000 Jun 15;19(12):2877-88. PMID:10856233
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