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1h9g

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{{STRUCTURE_1h9g| PDB=1h9g | SCENE= }}
{{STRUCTURE_1h9g| PDB=1h9g | SCENE= }}
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'''FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI, IN COMPLEX WITH MYRISTOYL-COA'''
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===FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI, IN COMPLEX WITH MYRISTOYL-COA===
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==Overview==
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FadR is an acyl-CoA-responsive transcription factor, regulating fatty acid biosynthetic and degradation genes in Escherichia coli. The apo-protein binds DNA as a homodimer, an interaction that is disrupted by binding of acyl-COA: The recently described structure of apo-FadR shows a DNA binding domain coupled to an acyl-CoA binding domain with a novel fold, but does not explain how binding of the acyl-CoA effector molecule &gt; 30 A away from the DNA binding site affects transcriptional regulation. Here, we describe the structures of the FadR-operator and FadR- myristoyl-CoA binary complexes. The FadR-DNA complex reveals a novel winged helix-turn-helix protein-DNA interaction, involving sequence-specific contacts from the wing to the minor groove. Binding of acyl-CoA results in dramatic conformational changes throughout the protein, with backbone shifts up to 4.5 A. The net effect is a rearrangement of the DNA binding domains in the dimer, resulting in a change of 7.2 A in separation of the DNA recognition helices and the loss of DNA binding, revealing the molecular basis of acyl-CoA-responsive regulation.
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{{ABSTRACT_PUBMED_11296236}}
==About this Structure==
==About this Structure==
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[[Category: Knudsen, J.]]
[[Category: Knudsen, J.]]
[[Category: Transcriptional regulation]]
[[Category: Transcriptional regulation]]
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Revision as of 04:50, 1 July 2008

Image:1h9g.png

Template:STRUCTURE 1h9g

FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI, IN COMPLEX WITH MYRISTOYL-COA

Template:ABSTRACT PUBMED 11296236

About this Structure

1H9G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR., van Aalten DM, DiRusso CC, Knudsen J, EMBO J. 2001 Apr 17;20(8):2041-50. PMID:11296236

Page seeded by OCA on Tue Jul 1 07:50:10 2008

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