3qfq

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Current revision (11:44, 14 March 2024) (edit) (undo)
 
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<StructureSection load='3qfq' size='340' side='right'caption='[[3qfq]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='3qfq' size='340' side='right'caption='[[3qfq]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qfq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mcpyv_isolate_r17b Mcpyv isolate r17b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QFQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qfq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Merkel_cell_polyomavirus Merkel cell polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QFQ FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qfq OCA], [https://pdbe.org/3qfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qfq RCSB], [https://www.ebi.ac.uk/pdbsum/3qfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qfq ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9001&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qfq OCA], [https://pdbe.org/3qfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qfq RCSB], [https://www.ebi.ac.uk/pdbsum/3qfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qfq ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/E2IPT4_9POLY E2IPT4_9POLY]
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The double-stranded DNA polyomavirus Merkel cell polyomavirus (MCV) causes Merkel cell carcinoma, an aggressive but rare human skin cancer that most often affects immunosuppressed and elderly persons. As in other polyomaviruses, the large T-antigen of MCV recognizes the viral origin of replication by binding repeating G(A/G)GGC pentamers. The spacing, number, orientation, and necessity of repeats for viral replication differ, however, from other family members such as SV40 and murine polyomavirus. We report here the 2.9-A crystal structure of the MCV large T-antigen origin binding domain (OBD) in complex with a DNA fragment from the MCV origin of replication. Consistent with replication data showing that three of the G(A/G)GGC-like binding sites near the center of the origin are required for replication, the crystal structure contains three copies of the OBD. This stoichiometry was verified using isothermal titration calorimetry. The affinity for G(A/G)GGC-containing double-stranded DNA was found to be approximately 740 nM, approximately 8-fold weaker than the equivalent domain in SV40 for the analogous region of the SV40 origin. The difference in affinity is partially attributable to DNA-binding residue Lys331 (Arg154 in SV40). In contrast to SV40, a small protein-protein interface is observed between MCV OBDs when bound to the central region of the origin. This protein-protein interface is reminiscent of that seen in bovine papilloma virus E1 protein. Mutational analysis indicates, however, that this interface contributes little to DNA binding energy.
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Asymmetric Assembly of Merkel Cell Polyomavirus Large T-Antigen Origin Binding Domains at the Viral Origin.,Harrison CJ, Meinke G, Kwun HJ, Rogalin H, Phelan PJ, Bullock PA, Chang Y, Moore PS, Bohm A J Mol Biol. 2011 Apr 9. PMID:21501625<ref>PMID:21501625</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3qfq" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Large T Antigen|Large T Antigen]]
*[[Large T Antigen|Large T Antigen]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Mcpyv isolate r17b]]
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[[Category: Merkel cell polyomavirus]]
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[[Category: Bohm, A]]
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[[Category: Bohm A]]
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[[Category: Harrison, C J]]
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[[Category: Harrison CJ]]
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[[Category: Meinke, G]]
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[[Category: Meinke G]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Origin binding domain]]
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[[Category: Protein-dna complex]]
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[[Category: Replication]]
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Current revision

Asymmetric Assembly of Merkel Cell Polyomavirus Large T-antigen Origin Binding Domains at the Viral Origin

PDB ID 3qfq

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