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3qq4

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Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides

Structural highlights

3qq4 is a 3 chain structure with sequence from Sudan ebolavirus and Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.097Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O19244_PIG Involved in the presentation of foreign antigens to the immune system.[SAAS:SAAS00058813]

Publication Abstract from PubMed

The presentation of viral epitopes to cytotoxic T lymphocytes (CTLs) by swine leukocyte antigen class I (SLA I) is crucial for swine immunity. To illustrate the structural basis of swine CTL epitope presentation, the first SLA crystal structures, SLA-1 0401, complexed with peptides derived from either 2009 pandemic H1N1 (pH1N1) swine-origin influenza A virus (S-OIV(NW9); NSDTVGWSW) or Ebola virus (Ebola(AY9); ATAAATEAY) were determined in this study. The overall peptide-SLA-1 0401 structures resemble, as expected, the general conformations of other structure-solved peptide major histocompatibility complexes (pMHC). The major distinction of SLA-1 0401 is that Arg(156) has a "one-ballot veto" function in peptide binding, due to its flexible side chain. S-OIV(NW9) and Ebola(AY9) bind SLA-1 0401 with similar conformations but employ different water molecules to stabilize their binding. The side chain of P7 residues in both peptides is exposed, indicating that the epitopes are "featured" peptides presented by this SLA. Further analyses showed that SLA-1 0401 and human leukocyte antigen (HLA) class I HLA-A 0101 can present the same peptides, but in different conformations, demonstrating cross-species epitope presentation. CTL epitope peptides derived from 2009 pandemic S-OIV were screened and evaluated by the in vitro refolding method. Three peptides were identified as potential cross-species influenza virus (IV) CTL epitopes. The binding motif of SLA-1 0401 was proposed, and thermostabilities of key peptide-SLA-1 0401 complexes were analyzed by circular dichroism spectra. Our results not only provide the structural basis of peptide presentation by SLA I but also identify some IV CTL epitope peptides. These results will benefit both vaccine development and swine organ-based xenotransplantation.

Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides.,Zhang N, Qi J, Feng S, Gao F, Liu J, Pan X, Chen R, Li Q, Chen Z, Li X, Xia C, Gao GF J Virol. 2011 Nov;85(22):11709-24. Epub 2011 Sep 7. PMID:21900158^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang N, Qi J, Feng S, Gao F, Liu J, Pan X, Chen R, Li Q, Chen Z, Li X, Xia C, Gao GF. Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides. J Virol. 2011 Nov;85(22):11709-24. Epub 2011 Sep 7. PMID:21900158 doi:10.1128/JVI.05040-11

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qq4"

@@ Line 3: / Line 3: @@
 <StructureSection load='3qq4' size='340' side='right'caption='[[3qq4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3qq4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QQ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3qq4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sudan_ebolavirus Sudan ebolavirus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QQ4 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q94|1q94]], [[3qq3|3qq3]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.097&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PD1, SLA-1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG]), B2M ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qq4 OCA], [https://pdbe.org/3qq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3qq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qq4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/O19244_PIG O19244_PIG]] Involved in the presentation of foreign antigens to the immune system.[SAAS:SAAS00058813] [[https://www.uniprot.org/uniprot/B2MG_PIG B2MG_PIG]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system (By similarity).
+[https://www.uniprot.org/uniprot/O19244_PIG O19244_PIG] Involved in the presentation of foreign antigens to the immune system.[SAAS:SAAS00058813]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
 *[[MHC 3D structures|MHC 3D structures]]
+*[[MHC I 3D structures|MHC I 3D structures]]
 == References ==
 <references/>
@@ Line 28: / Line 28: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pig]]
+[[Category: Sudan ebolavirus]]
-[[Category: Chen, R]]
+[[Category: Sus scrofa]]
-[[Category: Chen, Z]]
+[[Category: Chen R]]
-[[Category: Gao, F]]
+[[Category: Chen Z]]
-[[Category: Gao, G F]]
+[[Category: Gao F]]
-[[Category: Li, Q]]
+[[Category: Gao GF]]
-[[Category: Li, X]]
+[[Category: Li Q]]
-[[Category: Pan, X]]
+[[Category: Li X]]
-[[Category: Qi, J]]
+[[Category: Pan X]]
-[[Category: Xia, C]]
+[[Category: Qi J]]
-[[Category: Zhang, N]]
+[[Category: Xia C]]
-[[Category: Epitope of ebola virus]]
+[[Category: Zhang N]]
-[[Category: Immune system]]
-[[Category: Sla-1*0401]]
-[[Category: Swine mhc class 1]]

3qq4

From Proteopedia

Current revision

Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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