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| | <StructureSection load='3rg8' size='340' side='right'caption='[[3rg8]], [[Resolution|resolution]] 1.74Å' scene=''> | | <StructureSection load='3rg8' size='340' side='right'caption='[[3rg8]], [[Resolution|resolution]] 1.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rg8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"spirillum_dentium"_(miller_1889)_sternberg_1892 "spirillum dentium" (miller 1889) sternberg 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RG8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rg8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_denticola Treponema denticola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RG8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PurE, TDE_0687 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158 "Spirillum dentium" (Miller 1889) Sternberg 1892])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoribosylaminoimidazole_carboxylase Phosphoribosylaminoimidazole carboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.21 4.1.1.21] </span></td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rg8 OCA], [https://pdbe.org/3rg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rg8 RCSB], [https://www.ebi.ac.uk/pdbsum/3rg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rg8 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rg8 OCA], [https://pdbe.org/3rg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rg8 RCSB], [https://www.ebi.ac.uk/pdbsum/3rg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rg8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/Q73PV9_TREDE Q73PV9_TREDE]] Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).[HAMAP-Rule:MF_01929][PIRNR:PIRNR001338]
| + | [https://www.uniprot.org/uniprot/PURE_TREDE PURE_TREDE] Catalyzes the reversible conversion of 5-aminoimidazole ribonucleotide (AIR) and CO(2) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR). Does not accept N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) as a substrate.<ref>PMID:21548610</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
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| - | De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires a single enzyme, AIR carboxylase (class II PurE). The CAIR carboxylate derives from bicarbonate or CO(2), respectively. Class I PurE is a promising antimicrobial target. Class I and class II PurEs are mechanistically related but bind different substrates. The spirochete dental pathogen Treponema denticola lacks a purK gene and contains a class II purE gene, the hallmarks of CO(2)-dependent CAIR synthesis. We demonstrate that T. denticola PurE (TdPurE) is AIR carboxylase, the first example of a prokaryotic class II PurE. Steady-state and pre-steady-state experiments show that TdPurE binds AIR and CO(2) but not N(5)-CAIR. Crystal structures of TdPurE alone and in complex with AIR show a conformational change in the key active site His40 residue that is not observed for class I PurEs. A contact between the AIR phosphate and a differentially conserved residue (TdPurE Lys41) enforces different AIR conformations in each PurE class. As a consequence, the TdPurE.AIR complex contains a portal that appears to allow the CO(2) substrate to enter the active site. In the human pathogen T. denticola, purine biosynthesis should depend on available CO(2) levels. Because spirochetes lack carbonic anhydrase, the corresponding reduction in bicarbonate demand may confer a selective advantage.
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| - | Treponema denticola PurE Is a Bacterial AIR Carboxylase.,Tranchimand S, Starks CM, Mathews II, Hockings SC, Kappock TJ Biochemistry. 2011 May 6. PMID:21548610<ref>PMID:21548610</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3rg8" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phosphoribosylaminoimidazole carboxylase]] | + | [[Category: Treponema denticola]] |
| - | [[Category: Kappock, T J]] | + | [[Category: Kappock TJ]] |
| - | [[Category: Mathews, I I]] | + | [[Category: Mathews II]] |
| - | [[Category: Starks, C M]] | + | [[Category: Starks CM]] |
| - | [[Category: Carboxylase]]
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| - | [[Category: Lyase]]
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| - | [[Category: Purine biosynthesis]]
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