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1hd3

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{{STRUCTURE_1hd3| PDB=1hd3 | SCENE= }}
{{STRUCTURE_1hd3| PDB=1hd3 | SCENE= }}
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'''A-SPECTRIN SH3 DOMAIN F52Y MUTANT'''
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===A-SPECTRIN SH3 DOMAIN F52Y MUTANT===
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==Overview==
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Here we present a method for determining the inference of non-native conformations in the folding of a small domain, alpha-spectrin Src homology 3 domain. This method relies on the preservation of all native interactions after Tyr/Phe exchanges in solvent-exposed, contact-free positions. Minor changes in solvent exposure and free energy of the denatured ensemble are in agreement with the reverse hydrophobic effect, as the Tyr/Phe mutations slightly change the polypeptide hydrophilic/hydrophobic balance. Interestingly, more important Gibbs energy variations are observed in the transition state ensemble (TSE). Considering the small changes induced by the H/OH replacements, the observed energy variations in the TSE are rather notable, but of a magnitude that would remain undetected under regular mutations that alter the folded structure free energy. Hydrophobic residues outside of the folding nucleus contribute to the stability of the TSE in an unspecific nonlinear manner, producing a significant acceleration of both unfolding and refolding rates, with little effect on stability. These results suggest that sectors of the protein transiently reside in non-native areas of the landscape during folding, with implications in the reading of phi values from protein engineering experiments. Contrary to previous proposals, the principle that emerges is that non-native contacts, or conformations, could be beneficial in evolution and design of some fast folding proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 11959988 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11959988}}
==About this Structure==
==About this Structure==
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[[Category: Cytoskeleton]]
[[Category: Cytoskeleton]]
[[Category: Sh3-domain]]
[[Category: Sh3-domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:43:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:00:52 2008''

Revision as of 05:00, 1 July 2008

Image:1hd3.png

Template:STRUCTURE 1hd3

A-SPECTRIN SH3 DOMAIN F52Y MUTANT

Template:ABSTRACT PUBMED 11959988

About this Structure

1HD3 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Unspecific hydrophobic stabilization of folding transition states., Viguera AR, Vega C, Serrano L, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5349-54. PMID:11959988

Page seeded by OCA on Tue Jul 1 08:00:52 2008

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