7uiu
From Proteopedia
(Difference between revisions)
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==N2 sub-domain of IF2 bound to the 30S subunit in the Pseudomonas aeruginosa 70S ribosome initiation complex (focused classification and refinement)== | ==N2 sub-domain of IF2 bound to the 30S subunit in the Pseudomonas aeruginosa 70S ribosome initiation complex (focused classification and refinement)== | ||
| - | <StructureSection load='7uiu' size='340' side='right'caption='[[7uiu]]' scene=''> | + | <StructureSection load='7uiu' size='340' side='right'caption='[[7uiu]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UIU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7uiu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UIU FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uiu OCA], [https://pdbe.org/7uiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uiu RCSB], [https://www.ebi.ac.uk/pdbsum/7uiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uiu ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uiu OCA], [https://pdbe.org/7uiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uiu RCSB], [https://www.ebi.ac.uk/pdbsum/7uiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uiu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/IF2_PSEAE IF2_PSEAE]] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.[HAMAP-Rule:MF_00100] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-tRNAi(fMet)) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit joining IF2 hydrolyzes GTP and, concomitant with inorganic phosphate (Pi) release, changes conformation facilitating fMet-tRNAi(fMet) accommodation into the P site and transition of the 70 S ribosome initiation complex (70S-IC) to an elongation-competent ribosome. The mechanism by which IF2 separates from initiator tRNA at the end of translation initiation remains elusive. Here, we report cryo-electron microscopy (cryo-EM) structures of the 70S-IC from Pseudomonas aeruginosa bound to compact IF2-GDP and initiator tRNA. Relative to GTP-bound IF2, rotation of the switch 2 alpha-helix in the G-domain bound to GDP unlocks a cascade of large-domain movements in IF2 that propagate to the distal tRNA-binding domain C2. The C2-domain relocates 35 angstroms away from tRNA, explaining how IF2 makes way for fMet-tRNAi(fMet) accommodation into the P site. Our findings provide the basis by which IF2 gates the ribosome to the elongation phase. | ||
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| + | Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation.,Basu RS, Sherman MB, Gagnon MG Nat Commun. 2022 Jun 13;13(1):3388. doi: 10.1038/s41467-022-31129-2. PMID:35697706<ref>PMID:35697706</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7uiu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Basu | + | [[Category: Basu, R S]] |
| - | [[Category: Gagnon | + | [[Category: Gagnon, M G]] |
| - | [[Category: Sherman | + | [[Category: Sherman, M B]] |
| + | [[Category: 70s ribosome]] | ||
| + | [[Category: Conformational change]] | ||
| + | [[Category: Cryo-em]] | ||
| + | [[Category: Initiation factor 2]] | ||
| + | [[Category: Initiator trna]] | ||
| + | [[Category: Ribosome]] | ||
| + | [[Category: Translation initiation]] | ||
Revision as of 07:35, 29 June 2022
N2 sub-domain of IF2 bound to the 30S subunit in the Pseudomonas aeruginosa 70S ribosome initiation complex (focused classification and refinement)
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