7w5a

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Current revision (06:19, 3 July 2025) (edit) (undo)
 
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====
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==The cryo-EM structure of human pre-C*-II complex==
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<StructureSection load='7w5a' size='340' side='right'caption='[[7w5a]]' scene=''>
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<StructureSection load='7w5a' size='340' side='right'caption='[[7w5a]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7w5a]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W5A FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w5a OCA], [https://pdbe.org/7w5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w5a RCSB], [https://www.ebi.ac.uk/pdbsum/7w5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w5a ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w5a OCA], [https://pdbe.org/7w5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w5a RCSB], [https://www.ebi.ac.uk/pdbsum/7w5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w5a ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RBM22_HUMAN RBM22_HUMAN] Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.<ref>PMID:17045351</ref> <ref>PMID:21122810</ref> <ref>PMID:22246180</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pre-mRNA splicing involves two sequential reactions: branching and exon ligation. The C complex after branching undergoes remodeling to become the C( *) complex, which executes exon ligation. Here, we report cryo-EM structures of two intermediate human spliceosomal complexes, pre-C( *)-I and pre-C( *)-II, both at 3.6 A. In both structures, the 3' splice site is already docked into the active site, the ensuing 3' exon sequences are anchored on PRP8, and the step II factor FAM192A contacts the duplex between U2 snRNA and the branch site. In the transition of pre-C( *)-I to pre-C( *)-II, the step II factors Cactin, FAM32A, PRKRIP1, and SLU7 are recruited. Notably, the RNA helicase PRP22 is positioned quite differently in the pre-C( *)-I, pre-C( *)-II, and C( *) complexes, suggesting a role in 3' exon binding and proofreading. Together with information on human C and C( *) complexes, our studies recapitulate a molecular choreography of the C-to-C( *) transition, revealing mechanistic insights into exon ligation.
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Mechanism of exon ligation by human spliceosome.,Zhan X, Lu Y, Zhang X, Yan C, Shi Y Mol Cell. 2022 Aug 4;82(15):2769-2778.e4. doi: 10.1016/j.molcel.2022.05.021. Epub , 2022 Jun 14. PMID:35705093<ref>PMID:35705093</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7w5a" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Z-disk]]
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[[Category: Lu Y]]
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[[Category: Shi Y]]
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[[Category: Zhan X]]

Current revision

The cryo-EM structure of human pre-C*-II complex

PDB ID 7w5a

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