This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3s2u

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:47, 1 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='3s2u' size='340' side='right'caption='[[3s2u]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
<StructureSection load='3s2u' size='340' side='right'caption='[[3s2u]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3s2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S2U FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3s2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S2U FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">murG, PA4412 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Undecaprenyldiphospho-muramoylpentapeptide_beta-N-_acetylglucosaminyltransferase Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.227 2.4.1.227] </span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s2u OCA], [https://pdbe.org/3s2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s2u RCSB], [https://www.ebi.ac.uk/pdbsum/3s2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s2u ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s2u OCA], [https://pdbe.org/3s2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s2u RCSB], [https://www.ebi.ac.uk/pdbsum/3s2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s2u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/MURG_PSEAE MURG_PSEAE]] Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
+
[https://www.uniprot.org/uniprot/MURG_PSEAE MURG_PSEAE] Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
MurG is an essential bacterial glycosyltransferase enzyme in Pseudomonas aeruginosa performing one of the key membrane steps of peptidoglycan synthesis catalyzing the transfer of N-acetyl glucosamine (GlcNAc) from its donor substrate, UDP-GlcNAc, to the acceptor substrate Lipid I. We have solved the crystal structure of the complex between Pseudomonas aeruginosa MurG and UDP-GlcNAc and compared it with the previously solved complex from E. coli. The structure reveals a large-scale conformational change in the relative orientations of the N- and C-terminal domains, which has the effect of widening the cofactor binding site and displacing the UDP-GlcNAc donor. These results suggest new opportunities to design potent inhibitors of peptidoglycan biosynthesis.
+
-
 
+
-
Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex.,Brown K, Vial SC, Dedi N, Westcott J, Scally S, Bugg TD, Charlton PA, Cheetham GM Protein Pept Lett. 2012 Sep 4. PMID:22973843<ref>PMID:22973843</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3s2u" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
-
== References ==
 
-
<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Pseae]]
+
[[Category: Pseudomonas aeruginosa PAO1]]
-
[[Category: Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase]]
+
[[Category: Brown K]]
-
[[Category: Brown, K]]
+
[[Category: Bugg TDH]]
-
[[Category: Bugg, T D.H]]
+
[[Category: Charlton PA]]
-
[[Category: Charlton, P A]]
+
[[Category: Cheetham GMT]]
-
[[Category: Cheetham, G M.T]]
+
[[Category: Dedi N]]
-
[[Category: Dedi, N]]
+
[[Category: Scally S]]
-
[[Category: Scally, S]]
+
[[Category: Vial SCM]]
-
[[Category: Vial, S C.M]]
+
[[Category: Westcott J]]
-
[[Category: Westcott, J]]
+
-
[[Category: N-acetylglucosaminyl transferase]]
+
-
[[Category: Transferase]]
+

Current revision

Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex

PDB ID 3s2u

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3s2u"
Personal tools