1h5z
From Proteopedia
(New page: 200px<br /> <applet load="1h5z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h5z, resolution 2.05Å" /> '''CYTOCHROME P450 14 ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H5Z is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with FE2 and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5Z OCA]]. | + | 1H5Z is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with FE2 and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Sterol_14-demethylase Sterol 14-demethylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.70 1.14.13.70]]. Structure known Active Site: HEM. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5Z OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Sterol 14-demethylase]] | ||
[[Category: Arase, M.]] | [[Category: Arase, M.]] | ||
[[Category: Podust, L.M.]] | [[Category: Podust, L.M.]] | ||
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[[Category: sterol biosynthesis]] | [[Category: sterol biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:35:54 2007'' |
Revision as of 09:31, 30 October 2007
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CYTOCHROME P450 14 ALPHA-STEROL DEMETHYLASE (CYP51) FROM MYCOBACTERIUM TUBERCULOSIS IN FERRIC LOW-SPIN STATE
Overview
Sterol 14alpha-demethylases (CYP51) are essential enzymes in sterol, biosynthesis in eukaryotes and drug targets in antifungal therapy. Here, we report CYP51 structures in ligand-free and estriol bound forms. Using, estriol as a probe, we determined orientation of the substrate in the, active site, elucidated protein contacts with the invariant 3beta-hydroxy, group of a sterol, and identified F78 as a key discriminator between, 4alpha-methylated and 4alpha,beta-dimethylated substrates. Analysis of, CYP51 dynamics revealed that the C helix undergoes helix-coil transition, upon binding and dissociation of a ligand. Loss of helical structure of, the C helix in the ligand-free form results in an unprecedented opening of, the substrate binding site. Upon binding of estriol, the BC loop loses, ... [(full description)]
About this Structure
1H5Z is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with FE2 and HEM as [ligands]. Active as [Sterol 14-demethylase], with EC number [1.14.13.70]. Structure known Active Site: HEM. Full crystallographic information is available from [OCA].
Reference
Estriol bound and ligand-free structures of sterol 14alpha-demethylase., Podust LM, Yermalitskaya LV, Lepesheva GI, Podust VN, Dalmasso EA, Waterman MR, Structure. 2004 Nov;12(11):1937-45. PMID:15530358
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